Quantitative analysis of allelic differences in the grain proteome between theWx(g2)andWx(g3)alleles in rice (Oryza sativaL.)

文献类型： 外文期刊

第一作者： Teng, Bin

作者： Teng, Bin;Zhang, Ying;Zhang, Chen

作者机构：

关键词： Rice; Wxgene; Allelic variation; Quantitative proteomic; Single-segment substitution line

期刊名称：EUPHYTICA （ 影响因子：1.895； 五年影响因子：2.181 ）

ISSN： 0014-2336

年卷期： 2020 年 216 卷 9 期

页码：

收录情况： SCI

摘要： TheWxgene is the major gene controlling amylose synthesis in rice endosperm. In a previous study, we were able to separate theWx(a)allele into two functionalWxalleles,Wx(g2)andWx(g3), according to their amylose phenotypes and a T/C nucleotide polymorphism in exon 10. Subsequent studies revealed that thisWxallelic variation also plays a vital role in regulating other important starch properties. However, the underlying molecular mechanisms related to the rice grain proteome have remained unclear. To elucidate this, we used two rice single-segment substitution lines harboring differentWxalleles,Wx(g2)andWx(g3), in comparative proteomic studies. On the basis of iTRAQ quantitative proteomics, we identified a total of 185 proteins as differentially accumulated between theWx(g2)andWx(g3)lines. Gene Ontology functional analysis revealed that a high proportion of differentially expressed proteins (DEPs) are involved in metabolic and cellular biological processes. Further functional and pathway enrichment analyses indicated that relevant functions of the DEPs on protein degradation and binding may play important roles in distinguishing theWx(g2)andWx(g3)alleles. The present results may provide comprehensive insights into the molecular regulatory mechanisms of starch biosynthesis as influenced by theWx(g2)andWx(g3)alleles in rice.

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