Molecular identification and developmental expression patterns of growth hormone and its receptors in yellowtail kingfish (Seriola lalandi)

文献类型： 外文期刊

第一作者： Wang, Bin

作者： Wang, Bin;Zhang, Yaxing;Liu, Quan;Xu, Yongjiang;Cui, Aijun;Jiang, Yan;Liu, Xuezhou;Wang, Bin;Xu, Yongjiang;Jiang, Yan;Liu, Xuezhou;Zhang, Yaxing;Liu, Quan;Cui, Aijun

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关键词： Growth hormone; Growth hormone receptor; Embryonic development; Seriola lalandi

期刊名称：MOLECULAR BIOLOGY REPORTS （ 影响因子：2.316； 五年影响因子：2.357 ）

ISSN： 0301-4851

年卷期： 2020 年 47 卷 9 期

页码：

收录情况： SCI

摘要： In fish and other vertebrates, growth hormone (GH) is an essential polypeptide required for normal growth and development. In an attempt to understand growth regulation in yellowtail kingfish (YTK), the full-length cDNA sequences encodingghand its receptors (ghr1andghr2) were cloned, characterized and the expression profiles of these three genes were investigated during embryonic development. The full-length cDNA sequences of GH and its receptors were obtained by RT-PCR combined with RACE methord. YTKghcDNA sequence was 852 base pairs (bp) that comprised an open reading frame (ORF) of 615 bp encoding a 204-amino acids (aa) precursor. The preprohormone compassed a signal peptide (17 aa) and the mature peptide (187 aa). YTK GHR1 protein consisted of a signal peptide (28 aa), an extracellular domain (222 aa), a single transmembrane domain (23 aa) and an intracellular domain (361 aa). GHR2 protein included 18 aa, 223 aa, 23 aa, and 321 aa, respectively. Tissue distribution analysis showed that the maximal level ofghexpression was observed in the pituitary, andghr1mRNA was mainly detected in the liver, whileghr2transcripts were most abundant in the gonad. Moreover, bothghr1andghr2mRNAs were expressed in all embryonic stages and displayed different gene expression profiles. Overall, these results provide initial evidences for the involvement of the GH/GHR system in the early ontogeny of yellowtail kingfish.

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