Integrative transcriptome and proteome analyses provide new insights into different stages ofAkebia trifoliatafruit cracking during ripening

文献类型： 外文期刊

第一作者： Niu, Juan

作者： Niu, Juan;Shi, Yaliang;Huang, Kunyong;Zhong, Yicheng;Chen, Jing;Sun, Zhimin;Luan, Mingbao;Chen, Jianhua

作者机构：

关键词： Akebia trifoliata; Pericarp structure; Fruit cracking; Transcriptome; Proteome; Pentose and glucuronate interconversions; Galactose metabolism

期刊名称：BIOTECHNOLOGY FOR BIOFUELS （ 影响因子：6.04； 五年影响因子：6.485 ）

ISSN：

年卷期： 2020 年 13 卷 1 期

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收录情况： SCI

摘要： Background Akebia trifoliata(Thunb.) Koidz may have applications as a new potential source of biofuels owing to its high seed count, seed oil content, and in-field yields. However, the pericarp ofA. trifoliatacracks longitudinally during fruit ripening, which increases the incidence of pests and diseases and can lead to fruit decay and deterioration, resulting in significant losses in yield. Few studies have evaluated the mechanisms underlyingA. trifoliatafruit cracking. Results In this study, by observing the cell wall structure of the pericarp, we found that the cell wall became thinner and looser and showed substantial breakdown in the pericarp of cracking fruit compared with that in non-cracking fruit. Moreover, integrative analyses of transcriptome and proteome profiles at different stages of fruit ripening demonstrated changes in the expression of various genes and proteins after cracking. Furthermore, the mRNA levels of 20 differentially expressed genes were analyzed, and parallel reaction monitoring analysis of 20 differentially expressed proteins involved in cell wall metabolism was conducted. Among the molecular targets, pectate lyases and pectinesterase, which are involved in pentose and glucuronate interconversion, and beta-galactosidase 2, which is involved in galactose metabolism, were significantly upregulated in cracking fruits than in non-cracking fruits. This suggested that they might play crucial roles inA. trifoliatafruit cracking. Conclusions Our findings provided new insights into potential genes influencing the fruit cracking trait inA. trifoliataand established a basis for further research on the breeding of cracking-resistant varieties to increase seed yields for biorefineries.

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