FusariumSpecies andFusarium oxysporumSpecies Complex Genotypes Associated With Yam Wilt in South-Central China

文献类型： 外文期刊

第一作者： Dongzhen, Fang

作者： Dongzhen, Fang;Xilin, Liu;Yunlu, He;Yi, Cheng;Jia, Chen;Zhimin, Li;Litao, Guo;Tuhong, Wang;Chunsheng, Gao;Dongzhen, Fang;Xilin, Liu;Yunlu, He;Yi, Cheng;Jia, Chen;Zhimin, Li;Litao, Guo;Tuhong, Wang;Chunsheng, Gao;Dongzhen, Fang;Xilin, Liu;Yunlu, He;Yi, Cheng;Jia, Chen;Zhimin, Li;Litao, Guo;Tuhong, Wang;Chunsheng, Gao;Xiaorong, Chen;Wenwu, Yan;Xu, Jianping

作者机构：

关键词： yam (DioscoreaL; ); yam wilt; host-pathogen association; multiple infections; multilocus microsatellite genotyping; gene flow; geographic differentiation

期刊名称：FRONTIERS IN MICROBIOLOGY （ 影响因子：5.64； 五年影响因子：6.32 ）

ISSN： 1664-302X

年卷期： 2020 年 11 卷

页码：

收录情况： SCI

摘要： Chinese yam (Dioscorea polystachyaThunb.) is an important root crop. Wilt caused byFusariumis among the most important emerging diseases on yams. However, there is currently limited information on the molecular epidemiology ofFusariumcausing yam wilt. Here, we investigated wilted yam samples from six regions in South-Central China. A total of 117Fusariumisolates were obtained from diseased tissues of 37 wilted yam plants. These yam plants belonged to two varieties characterized by white and purple fleshy tubers, respectively. Analyses ofef1-alpha sequences identified that these 117Fusariumisolates belonged to 11 putative species, withF. aff. communebeing the most common (31.6%), followed byF. aff. cugenangense(29.1%), a potential undescribed speciesFusarium aff. sp. (11.1%),F. aff. gossypinum(9.4%),F. aff. fujikuroi(8.5%),F. aff. nirenbergiae(6%), and one isolate each (0.85%) ofF. aff. asiaticum,F. aff. curvatum,F. aff. odoratissimum, F. aff. solani, andF. aff. verticillioides. Six of these species were recently described as new species within theFusarium oxysporumspecies complex (FOSC). Interestingly, 18 of the 37 yam plants were infected by two or moreFusariumspecies each and there was evidence for differentialFusariumspecies distributions based on geographic location and/or yam host variety. Multilocus microsatellite genotyping of the 67 FOSC isolates revealed that isolates of the same species from the same diseased plants often belonged to different genotypes. Interestingly, several FOSC microsatellite genotypes were shared among distinct geographic regions, consistent with long-distance dispersal. However, population genetic analyses revealed significant contributions of geographic separation to the overall genetic variation of FOSC with several pairs of geographic populations showing significant genetic differentiations, consistent with differential geographic distribution of the species within FOSC. The implications of our results to the managements ofFusariumwilt in yams were discussed.

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