A Simple and Effective Technique for Production of Pycnidia and Pycnidiospores by Macrophomina phaseolina

文献类型： 外文期刊

第一作者： Zhao, Xinbei

作者： Zhao, Xinbei;Ni, Yunxia;Liu, Xintao;Zhao, Hui;Wang, Jing;Liu, Hongyan;Chen, Yung-Chun;Chen, Weidong

作者机构：

关键词： Macrophomina phaseolina; oatmeal agar; ultraviolet light; sporulation; pathogenicity

期刊名称：PLANT DISEASE （ 影响因子：4.438； 五年影响因子：4.7 ）

ISSN： 0191-2917

年卷期： 2020 年 104 卷 4 期

页码：

收录情况： SCI

摘要： Production of pycnidia and pycnidiospores by Macrophomina phaseolina is not often seen in vitro. The objective of this study is to develop a simple and effective technique to obtain pycnidiospores of M. phaseolina isolates in vitro and to evaluate germination rates and pathogenicity of pycnidiospores. We found M. phaseolina isolates can produce pycnidia on oatmeal agar (OMA) under ultraviolet light with 365 nm wavelength (UV). For evaluating the effect of OMA and UV on growth of M. phaseolina, combinations of two agar media and three lighting conditions were tested. The results confirm that all six M. phaseolina isolates produced pycnidia only on OMA under UV. The pycnidiospores produced on OMA under UV had germination rates higher than 90%. In pathogenicity tests, inoculation with the pycnidiospores showed symptoms later than inoculation with hypha-colonized toothpicks. Significant differences in the pathogenicity is detected between isolates Mp2014003 and Mp2014024 when inoculation is done with the pycnidiospores (P < 0.001), but not when hypha-colonized toothpicks are used as inoculum (P = 0.091). This study provides a new method for obtaining pycnidiospores of M. phaseolina for future investigations.

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