Purification and characteristics of individual major royal jelly protein 1-3

文献类型： 外文期刊

第一作者： Wang, Xueyu

作者： Wang, Xueyu;Dong, Jie;Qiao, Jiangtao;Zhang, Hongcheng;Zhang, Gensheng;Dong, Jie;Zhang, Hongcheng

作者机构：

关键词： MRJPs; purity; yield; MRJP1 oligomers; secondary structure

期刊名称：JOURNAL OF APICULTURAL RESEARCH （ 影响因子：2.584； 五年影响因子：2.52 ）

ISSN： 0021-8839

年卷期： 2020 年 59 卷 5 期

页码：

收录情况： SCI

摘要： Major royal jelly proteins (MRJPs) are deemed to the most characteristic and abundant constituents in royal jelly. The present paper establishes a sequential process to purify the natural MRJP 1-3 by three different chromatographic procedures. Five individual proteins were obtained, including MRJP 1 oligomer 1, MRJP 1 oligomer 2, MRJP 1 monomer, MRJP 2 and MRJP 3s (contain three MRJP 3 variants) with a purity of 89.88%, 67.79%, 99.92%, 99.41%, and 99.97%, respectively. This is the first report that MRJP 1 mainly possesses three oligomers: a 228 kDa MRJP 1 oligomer 1, a 408 kDa MRJP 1 oligomer 2 and a 639 kDa MRJP 1 oligomer 3. We infer that MRJP 1 oligomer 3 (639 kDa) may be assembled by MRJP 1 oligomer 1 (228 kDa) and MRJP 1 oligomer 2 (408 kDa). The secondary structure of the purified MRJP 1-3 consisted predominantly of beta-sheets and random coil in the native conformation. Our results will contribute to the physiological and physicochemical function of the purified individual MRJPs.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序