Characterization of Nucleocytoplasmic Shuttling of Pseudorabies Virus Protein UL46

文献类型： 外文期刊

第一作者： Xu, Jing-jing

作者： Xu, Jing-jing;Gao, Fei;Wu, Ji-qiang;Zheng, Hao;Tong, Wu;Cheng, Xue-fei;Liu, Yuting;Zhu, Haojie;Fu, Xinling;Jiang, Yifeng;Li, Liwei;Kong, Ning;Li, Guoxin;Tong, Guangzhi;Li, Guoxin;Tong, Guangzhi

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关键词： pseudorabies virus; VP11; 12; nucleocytoplasmic shuttling; nuclear localization signal; VP16; EP0; STING

期刊名称：FRONTIERS IN VETERINARY SCIENCE （ 影响因子：3.412； 五年影响因子：3.588 ）

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年卷期： 2020 年 7 卷

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收录情况： SCI

摘要： Pseudorabies virus (PRV) is the etiological agent of Aujeszky's disease, which has caused severe economic loss in China since its re-emergence in 2011. UL46, a late gene of herpesvirus, codes for the abundant but non-essential viral phosphoproteins 11 and 12 (VP11/12). In this study, VP11/12 was found to localize inside both the nucleus and cytoplasm. The nuclear localization signal (NLS) of VP11/12 was identified as(3)RRARGTRRASWKDASR(18). Further research identified alpha 5 and alpha 7 to be the receptors for NLS and the chromosome region maintenance 1 (CRM1) to be the receptor for the nuclear export signal. Moreover, we found that PRV VP11/12 interacts with EP0 and the stimulator of interferon genes protein (STING), whereas the NLS of VP11/12 is the important part for VP11/12 to interact with UL48. To our knowledge, this is the first study to provide reliable evidence verifying the nuclear localization of VP11/12 and its role as an additional shuttling tegument protein for PRV. In addition, this is also the first study to elucidate the interactions between PRV VP11/12 and EP0 as well as between PRV VP11/12 and STING, while identifying the precise interaction sites of PRV VP11/12 and VP16.

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