Genome-wide identification of the Sec-dependent secretory protease genes inErwinia amylovoraand analysis of their expression during infection of immature pear fruit

文献类型： 外文期刊

第一作者： Zhang, Wang-bin

作者： Zhang, Wang-bin;Yan, Hai-lin;Zhu, Zong-cai;Zhang, Wang-bin;Yan, Hai-lin;Zhu, Zong-cai;Zhang, Chao;Du, Pei-xiu;Li, Wei-min;Zhao, Wen-jun

作者机构：

关键词： Erwinia amylovora; Sec-dependent pathway; Protease; Gene expression; Plant infection; S432; 1

期刊名称：JOURNAL OF ZHEJIANG UNIVERSITY-SCIENCE B （ 影响因子：3.066； 五年影响因子：3.057 ）

ISSN： 1673-1581

年卷期： 2020 年 21 卷 9 期

页码：

收录情况： SCI

摘要： The general secretory (Sec) pathway represents a common mechanism by which bacteria secrete proteins, including virulence factors, into the extracytoplasmic milieu. However, there is little information about this system, as well as its associated secretory proteins, in relation to the fire blight pathogenErwinia amylovora. In this study, data mining revealed thatE. amylovoraharbors all of the essential components of the Sec system. Based on this information, we identified putative Sec-dependent secretory proteases inE. amylovoraon a genome-wide scale. Using the programs SignalP, LipoP, and Phobius, a total of 15 putative proteases were predicted to contain the N-terminal signal peptides (SPs) that might link them to the Sec-dependent pathway. The activities of the predicted SPs were further validated using anEscherichia coli-based alkaline phosphatase (PhoA) gene fusion system that confirmed their extracytoplasmic property. Transcriptional analyses showed that the expression of 11 of the 15 extracytoplasmic protease genes increased significantly whenE. amylovorawas used to inoculate immature pears, suggesting their potential roles in plant infection. The results of this study support the suggestion thatE. amylovoramight employ the Sec system to secrete a suite of proteases to enable successful infection of plants, and shed new light on the interaction ofE. amylovorawith host plants.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序