Immunological regulation by a beta-adrenergic-like octopamine receptor gene in crowded larvae of the oriental Armyworm, Mythmina separata

文献类型： 外文期刊

第一作者： Kong, Hailong

作者： Kong, Hailong;Yuan, Lin;Dong, Chuanlei;Zheng, Minyuan;Jing, Wanghui;Tian, Zhen;Hou, Qiuli;Cheng, Yunxia;Zhang, Lei;Jiang, Xingfu;Luo, Lizhi

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关键词： Prophylactic immunity; beta-adrenergic-like octopamine receptor; Mythimna separata; High density

期刊名称：DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY （ 影响因子：3.636； 五年影响因子：3.654 ）

ISSN： 0145-305X

年卷期： 2020 年 113 卷

页码：

收录情况： SCI

摘要： Recent reports demonstrate that octopamine plays an important immunological role in crowded larvae of the Oriental Armyworm, Mythmina separata. We identified an octopamine receptor, the beta-adrenergic-like gene (designated MsOctfl2R), with a 1191 bp open reading frame that encodes 396 amino acids and contains seven conserved hydrophobic transmembrane domains. Multiple sequence alignments and a phylogenetic analysis indicated that MsOctfl2R was orthologous to Octfl2R that is present in other lepidopterans. MsOctfl2R was expressed throughout all developmental stages with higher relative expression during the fourth instar and adult stages. MsOctfl2R was highly expressed in the ventral nerve cord and the fat body relative to other examined tissues. Elevated MsOctfl2R expression was observed in larvae that were under higher-density conditions (7 and 10 larvae per jar). Silencing MsOctfl2R expression via dsRNA injections in larvae from higher-density conditions significantly decreased phenoloxidase (PO) and lysozyme activity, total haemocyte counts, and survival rates against Beauveria bassiana infections (54.06%, 9.91%, 36.22%, and 23.53%, respectively) when compared with control larvae. These results suggest that high-density conditions might alter prophylactic immunity in larvae by regulating the MsOctfl2R gene in M. separara and provide new insights into density-dependent prophylaxis in insects.

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