verA Gene is Involved in the Step to Make the Xanthone Structure of Demethylsterigmatocystin in Aflatoxin Biosynthesis

文献类型： 外文期刊

第一作者： Zeng, Hongmei

作者： Zeng, Hongmei;Cai, Jingjing;Hatabayashi, Hidemi;Nakagawa, Hiroyuki;Yabe, Kimiko;Zeng, Hongmei;Cai, Jingjing;Nakajima, Hiromitsu;Yabe, Kimiko

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关键词： aflatoxin biosynthesis; enzyme gene; dmtA(aflO); hypA(aflY); ordB(aflX); HAMA intermediate; stcP; verA(aflN); ver-1(aflM)

期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：5.923； 五年影响因子：6.132 ）

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年卷期： 2020 年 21 卷 17 期

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收录情况： SCI

摘要： In the biosynthesis of aflatoxin,verA,ver-1,ordB, andhypAgenes of the aflatoxin gene cluster are involved in the pathway from versicolorin A (VA) to demethylsterigmatocystin (DMST). We herein isolated each disruptant of these four genes to determine their functions in more detail. Disruptants ofver-1,ordB, andhypAgenes commonly accumulated VA in their mycelia. In contrast, theverAgene disruptant accumulated a novel yellow fluorescent substance (which we named HAMA) in the mycelia as well as culture medium. Feeding HAMA to the other disruptants commonly caused the production of aflatoxins B-1(AFB(1)) and G(1)(AFG(1)). These results indicate that HAMA pigment is a novel aflatoxin precursor which is involved at a certain step after those ofver-1,ordB, andhypAgenes between VA and DMST. HAMA was found to be an unstable substance to easily convert to DMST and sterigmatin. A liquid chromatography-mass spectrometry (LC-MS) analysis showed that the molecular mass of HAMA was 374, and HAMA gave two close major peaks in the LC chromatogram in some LC conditions. We suggest that these peaks correspond to the two conformers of HAMA; one of them would be selectively bound on the substrate binding site of VerA enzyme and then converted to DMST. VerA enzyme may work as a key enzyme in the creation of the xanthone structure of DMST from HAMA.

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