Identification of a candidate gene associated with isoflavone content in soybean seeds using genome-wide association and linkage mapping

文献类型： 外文期刊

第一作者： Wu, Depeng

作者： Wu, Depeng;Li, Dongmei;Zhao, Xue;Zhan, Yuhang;Teng, Weili;Li, Wenbin;Han, Yingpeng;Qiu, Lijuan;Zheng, Hongkun

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关键词： soybean; isoflavone; GWAS; linkage maps; QTL; MPK

期刊名称：PLANT JOURNAL （ 影响因子：6.417； 五年影响因子：7.627 ）

ISSN： 0960-7412

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收录情况： SCI

摘要： Isoflavone, a secondary metabolite produced byGlycine max(L.) Merr. (soybean), is valuable for human and plant health. The genetic architecture of soybean isoflavone content remains unclear, however, despite several mapping studies. We generated genomic data for 200 soybean cultivars and 150 recombinant inbred lines (RILs) to localize putative loci associated with soybean seed isoflavone content. Using a genome-wide association study (GWAS), we identified 87 single-nucleotide polymorphisms (SNPs) that were significantly associated with isoflavone concentration. Using linkage mapping, we identified 37 quantitative trait loci (QTLs) underlying the content of four isoflavones found in the RILs. A major locus on chromosome 8 (qISO8-1) was co-located by both the GWAS and linkage mapping. qISO8-1 was fine mapped to a 99.5-kb region, flanked by SSR_08_1651 and SSR_08_1656, in a BC(2)F(5)population.GmMPK1, encoding a mitogen-activated protein kinase, was identified as the causal gene in qISO8-1, and two naturalGmMPK1polymorphisms were significantly associated with isoflavone content. Overexpression ofGmMPK1in soybean hairy roots resulted in increased isoflavone concentrations. OverexpressingGmMPK1in transgenic soybeans had greater resistance toPhytophthoraroot rot, suggesting thatGmMPK1might increase soybean resistance to biotic stress by influencing isoflavone content. Our results not only increase our understanding of the genetic architecture of soybean seed isoflavone content, but also provide a framework for the future marker-assisted breeding of high isoflavone content in soybean cultivars.

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