Parallel analysis of miRNAs and mRNAs suggests distinct regulatory networks inCrassostrea gigasinfected by Ostreid herpesvirus 1

文献类型： 外文期刊

第一作者： Rosani, Umberto

作者： Rosani, Umberto;Venier, Paola;Rosani, Umberto;Wegner, K. Mathias;Abbadi, Miriam;Arcangeli, Giuseppe;Green, Timothy;Green, Timothy;Bai, Chang-Ming;Turolla, Edoardo

作者机构：

关键词： Oyster; miRNA; OsHV-1; ADAR; C; gigas; miRNAome

期刊名称：BMC GENOMICS （ 影响因子：3.969； 五年影响因子：4.478 ）

ISSN： 1471-2164

年卷期： 2020 年 21 卷 1 期

页码：

收录情况： SCI

摘要： Background Since 2008, the aquaculture production ofCrassostrea gigaswas heavily affected by mass mortalities associated to Ostreid herpesvirus 1 (OsHV-1) microvariants worldwide. Transcriptomic studies revealed the major antiviral pathways of the oyster immune response while other findings suggested that also small non-coding RNAs (sncRNA) such as microRNAs might act as key regulators of the oyster response against OsHV-1. To explore the explicit connection between small non-coding and protein-coding transcripts, we performed paired whole transcriptome analysis of sncRNA and messenger RNA (mRNA) in six oysters selected for different intensities of OsHV-1 infection. Results The mRNA profiles of the naturally infected oysters were mostly governed by the transcriptional activity of OsHV-1, with several differentially expressed genes mapping to the interferon, toll, apoptosis, and pro-PO pathways. In contrast, miRNA profiles suggested more complex regulatory mechanisms, with 15 differentially expressed miRNAs (DE-miRNA) pointing to a possible modulation of the host response during OsHV-1 infection. We predicted 68 interactions between DE-miRNAs and oyster 3 '-UTRs, but only few of them involved antiviral genes. The sncRNA reads assigned to OsHV-1 rather resembled mRNA degradation products, suggesting the absence of genuine viral miRNAs. Conclusions We provided data describing the miRNAome during OsHV-1 infection inC. gigas. This information can be used to understand the role of miRNAs in healthy and diseased oysters, to identify new targets for functional studies and, eventually to disentangle cause and effect relationships during viral infections in marine mollusks.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序