PHOTO-SENSITIVE LEAF ROLLING 1encodes a polygalacturonase that modifies cell wall structure and drought tolerance in rice

文献类型： 外文期刊

第一作者： Zhang, Guangheng

作者： Zhang, Guangheng;Wang, Li;Xu, Jing;Fu, Xue;Shen, Nianwei;Nian, Jinqiang;Hu, Jiang;Zhu, Li;Rao, Yuchun;Ren, Deyong;Dong, Guojun;Gao, Zhenyu;Guo, Longbiao;Qian, Qian;Zhang, Guangheng;Hou, Xin;Luan, Sheng;Hou, Xin;Chen, Jian;Jiang, Zhuanzhuan;Shi, Yafei

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关键词： cell wall; drought tolerance; leaf morphogenesis; polygalacturonase (PG); rice (Oryza sativaL; )

期刊名称：NEW PHYTOLOGIST （ 影响因子：10.151； 五年影响因子：10.475 ）

ISSN： 0028-646X

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收录情况： SCI

摘要： The biosynthesis and modification of cell wall composition and structure are controlled by hundreds of enzymes and have a direct consequence on plant growth and development. However, the majority of these enzymes has not been functionally characterised. Rice mutants with leaf-rolling phenotypes were screened in a field. Phenotypic analysis under controlled conditions was performed for the selected mutant and the relevant gene was identified by map-based cloning. Cell wall composition was analysed by glycome profiling assay. We identified aphoto-sensitive leaf rolling 1(psl1) mutant with 'napping' (midday depression of photosynthesis) phenotype and reduced growth. ThePSL1gene encodes a cell wall-localised polygalacturonase (PG), a pectin-degrading enzyme.psl1with a 260-bp deletion in its gene displayed leaf rolling in response to high light intensity and/or low humidity. Biochemical assays revealed PG activity of recombinant PSL1 protein. Significant modifications to cell wall composition in thepsl1mutant compared with the wild-type plants were identified. Such modifications enhanced drought tolerance of the mutant plants by reducing water loss under osmotic stress and drought conditions. Taken together, PSL1 functions as a PG that modifies cell wall biosynthesis, plant development and drought tolerance in rice.

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