An electrochemical DNA biosensor based on nitrogen-doped graphene nanosheets decorated with gold nanoparticles for genetically modified maize detection

文献类型： 外文期刊

第一作者： Liu, Fuli

作者： Liu, Fuli;Zhang, Yukun;Ding, Jiaqi;Wen, Tingting;Ji, Wei;Liu, Jian;Zhang, Xiao;Li, Kai;Pei, Xinwu;Yan, Yongliang;Li, Liang

作者机构：

关键词： Genetically modified crops; Electrochemical biosensor; Nitrogen-doped graphene; Gold nanoparticles; Portable detection

期刊名称：MICROCHIMICA ACTA （ 影响因子：5.833； 五年影响因子：5.357 ）

ISSN： 0026-3672

年卷期： 2020 年 187 卷 10 期

页码：

收录情况： SCI

摘要： A reliable electrochemical biosensor is reported based on nitrogen-doped graphene nanosheets and gold nanoparticle (Au/N-G) nanocomposites for the event-specific detection of GM maize MIR162. The differential pulse voltammetry response of methylene blue (MB) was chosen to monitor the target DNA hybridization event. Under the optimum conditions, the peak current increased linearly with the logarithm of the concentration of DNA in the range 1.0 x 10(-14)to 1.0 x 10(-8) M, and the detection limit was 2.52 x 10(-15) M (S/N = 3). It is also demonstrated that the DNA biosensor has high selectivity, good stability, and fabrication reproducibility. The biosensor has been effectively applied to detect MIR162 in real samples, showing its potential as an effective tool for GM crop analysis. These results will contribute to the development of new portable transgenic detection systems. .

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