TaTCP-1, a Novel Regeneration-Related Gene Involved in the Molecular Regulation of Somatic Embryogenesis in Wheat (Triticum aestivumL.)

文献类型： 外文期刊

第一作者： Li, Feifei

作者： Li, Feifei;Li, Xiaoyan;Qiao, Meng;Li, Bo;Guo, Dongwei;Zhang, Xiaohong;Min, Donghong;Li, Feifei;Li, Xiaoyan;Li, Bo;Guo, Dongwei;Min, Donghong;Li, Feifei;Qiao, Meng;Zhang, Xiaohong

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关键词： callus regeneration frequency; gene transformation; somatic embryogenesis; TaTCP-1; wheat

期刊名称：FRONTIERS IN PLANT SCIENCE （ 影响因子：5.753； 五年影响因子：6.612 ）

ISSN： 1664-462X

年卷期： 2020 年 11 卷

页码：

收录情况： SCI

摘要： The lower regeneration rate of wheat calli is the main factor restricting the development of transgenic wheat plants. Therefore, improving the regeneration rate of wheat callus is a precondition for developing genetic engineering-based wheat breeding approaches. In the present study, we explored the molecular mechanism of wheat regeneration and aimed to establish an efficient system for transgenic wheat. We isolated and identified a regeneration-related gene,TaTCP-1(KC808517), from wheat cultivar Lunxuan 987. Sequence analysis revealed that the ORF ofTaTCP-1was 1623bp long encoding 540 amino acids. TheTaTCP-1gene was expressed in various wheat tissues. Further, the level ofTaTCP-1expression was higher in calli and increased gradually with increasing callus induction time, reaching a peak on the 11th day after induction. Moreover, the expression level ofTaTCP-1was higher in embryogenic calli than in non-embryonic calli. The TaTCP-1 protein was localized to the nucleus, cytoplasm, and cell membrane. The callus regeneration rate of wheat plants transformed with TaTCP-1-RNAi reduced by 85.09%. In contrast, it increased by 14.43% in plants overexpressingTaTCP-1. In conclusion, our results showed thatTaTCP-1played a vital role in promoting wheat regeneration, and regulated the somatic embryogenesis of wheat. These results may have implications in the genetic engineering of wheat for improved wheat production.

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