miR164-targetedTaPSK5encodes a phytosulfokine precursor that regulates root growth and yield traits in common wheat (Triticum aestivumL.)

文献类型： 外文期刊

第一作者： Geng, Yuke

作者： Geng, Yuke;Jian, Chao;Xu, Wu;Liu, Hong;Hao, Chenyang;Hou, Jian;Liu, Hongxia;Zhang, Xueyong;Li, Tian;Geng, Yuke

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关键词： miR164; TaPSK5; Root growth; Yield traits; Haplotype analysis; Wheat

期刊名称：PLANT MOLECULAR BIOLOGY （ 影响因子：4.076； 五年影响因子：4.89 ）

ISSN： 0167-4412

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收录情况： SCI

摘要： Key message TaPSK5is a less conserved target of miR164 in wheat encoding a positive regulator of root growth and yield traits that could be used for crop improvement. MicroRNAs (miRNAs) play key roles in regulating plant growth and development by targeting the mRNAs of conserved genes. However, little is known about the roles of less conserved miRNA-targeted genes in plants. In the current study, we identifiedTaPSK5, encoding a phytosulfokine precursor, as a novel target of miR164. Compared with miR164-targeted NAC transcription factor genes,TaPSK5is less conserved between monocots and dicots. Expression analysis indicated thatTaPSK5homoeologs were constitutively expressed in wheat tissues, especially young spikes. Overexpression ofTaPSK5-Dand miR164-resistantTaPSK5-D(r-TaPSK5-D) led to increased primary root growth and grain yield in rice, with the latter having more significant effects. Comparison of the transcriptome between wild-type andr-TaPSK5-Doverexpression plants revealed multiple differentially expressed genes involved in hormone signaling, transcription regulation, and reactive oxygen species (ROS) homeostasis. Moreover, we identified threeTaPSK5-Ahaplotypes (TaPSK5-A-Hap1/2/3) and twoTaPSK5-Bhaplotypes (TaPSK5-B-Hap1/2) in core collections of Chinese wheat. BothTaPSK5-A-Hap1andTaPSK5-B-Hap2are favorable haplotypes associated with superior yield traits that were under positive selection during wheat breeding. Together, our findings identify miR164-targetedTaPSK5as a regulator of root growth and yield traits in common wheat with potential applications for the genetic improvement of crops.

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