Targeting and signaling of Rho of plants guanosine triphosphatases require synergistic interaction between guanine nucleotide inhibitor and vesicular trafficking

文献类型： 外文期刊

第一作者： Ge, Fu-Rong

作者： Ge, Fu-Rong;Chai, Sen;Li, Sha;Zhang, Yan;Ge, Fu-Rong

作者机构：

关键词： GDI; prenylation; polarity; trans-Golgi network; early endosome; vesicle trafficking

期刊名称：JOURNAL OF INTEGRATIVE PLANT BIOLOGY （ 影响因子：7.061； 五年影响因子：6.002 ）

ISSN： 1672-9072

年卷期： 2020 年 62 卷 10 期

页码：

收录情况： SCI

摘要： Most eukaryotic cells are polarized. Common toolbox regulating cell polarization includes Rho guanosine triphosphatases (GTPases), in which spatiotemporal activation is regulated by a plethora of regulators. Rho of plants (ROPs) are the only Rho GTPases in plants. Although vesicular trafficking was hinted in the regulation of ROPs, it was unclear where vesicle-carried ROP starts, whether it is dynamically regulated, and which components participate in vesicle-mediated ROP targeting. In addition, although vesicle trafficking and guanine nucleotide inhibitor (GDI) pathways in Rho signaling have been extensively studied in yeast, it is unknown whether the two pathways interplay. Unclear are also cellular and developmental consequences of their interaction in multicellular organisms. Here, we show that the dynamic targeting of ROP through vesicles requires coat protein complex II and ADP-ribosylation factor 1-mediated post-Golgi trafficking. Trafficking of vesicle-carried ROPs between the plasma membrane and thetrans-Golgi network is mediated through adaptor protein 1 and sterol-mediated endocytosis. Finally, we show that GDI and vesicle trafficking synergistically regulate cell polarization and ROP targeting, suggesting that the establishment and maintenance of cell polarity is regulated by an evolutionarily conserved mechanism.

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