Effects of biochar on nitrification and denitrification-mediated N2O emissions and the associated microbial community in an agricultural soil

文献类型： 外文期刊

第一作者： Liu, Xingren

作者： Liu, Xingren;Shi, Yulong;Zhang, Qingwen;Li, Guichun

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关键词： Biochar; Isotope; Acetylene inhibition; High-throughput sequencing

期刊名称：ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH （ 影响因子：4.223； 五年影响因子：4.306 ）

ISSN： 0944-1344

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收录情况： SCI

摘要： Nitrous oxide (N2O) is a strong greenhouse gas, and it is of great significance for N2O reduction to study the effects of biochar on its production pathway. In this research, the contributions and mechanisms of biochar on autotrophic nitrification (ANF), heterotrophic nitrification (HNF), and denitrification (DF) to N2O emissions were studied by using(15)N stable isotopes and high-throughput sequencing after laboratory incubation. The results showed that biochar addition at 2% (B2) significantly reduced the N2O emissions from the ANF by an average of 20.6%, while adding 5% biochar (B5) had no significant effect on the ANF. Both B2 and B5 significantly reduced the N2O emissions from the HNF by 15.7% and 13.2%, respectively, and reduced the N2O emissions from the DF by 40.9% and 11.7%, respectively. B2 enhanced the relative contribution rate of the ANF to N2O emissions by 6.3%, while B5 had little effect on it. Biochar addition significantly changed the copy numbers of the AOA and AOB, as well as thenirK,nirS, andnosZgenes, but it had no significant effect on the community composition of the AOA and had minimal effect on the AOB community. B2 significantly increased the abundance of the genusRhodococcusofnirKtype denitrifiers and had a significant effect on the relative abundance ofCupriavidusandPseudomonasof thenosZtype denitrifiers. These results revealed that the inhibitory effects of biochar on N2O emissions from nitrification might be attributed to the direct immobilization and adsorption of inorganic N by biochar and to its promotion of the genusRhodococcusofnirK-type denitrifiers and the generaCupriavidusandPseudomonasof thenosZ-type denitrifiers. The soil exchangeable NH4+-N and NO3--N concentrations were the primary factors affecting the N2O emission rates. These results help to elucidate the effects and mechanisms of biochar on N2O production pathways in agricultural soil.

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