The biological characters ofBmelav-likegenes in the development ofBombyx mori

文献类型： 外文期刊

第一作者： Sun, X.

作者： Sun, X.;Zhang, K.;Gu, J.;Yang, J.;Huang, Q.;Yan, R.;Qin, S.;Hou, C.;Zhang, G.;Wang, S.;Li, M.;Sun, X.;Qin, S.;Hou, C.;Zhang, G.;Wang, S.;Li, M.

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关键词： RNA binding protein; gene expression; cocoon; oviposition; silkworms

期刊名称：INSECT MOLECULAR BIOLOGY （ 影响因子：3.585； 五年影响因子：3.215 ）

ISSN： 0962-1075

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收录情况： SCI

摘要： The ELAV/Hu family is a conserved multigene family of pan-neuronal RNA-binding protein involved in post-transcriptional regulation in metazoans. InDrosophila, three members of this family, ELAV, RBP9 and FNE, are involved in neuronal differentiation, gene expression regulation and so on. This family is less well characterized inBombyx mori. Two orthologs BmELAV-like-1 (BmEL-1) and BmELAV-like-2 (BmEL-2) share 55%-71% and 47%-62% identity with that of inDrosophilaand humans, respectively.Bmel-1is ubiquitously expressed whileBmel-2is expressed in the head and ovaries specifically. Proteins encoded by both genes are localized in nuclear and cytoplasm. The weight of body, cocoon, pupae and cocoon shell are differently affected inBmel-1(-)/-2(-)mutants created using CRISPR/Cas9 technology. Mutations of both genes increase the expression of four silk protein genes,Fib-L,Fib-H,P25andSer-1. In addition, the oviposition ability ofBmel-2(-)females is decreased. This study not only provides valuable insights into the functional roles ofBmelav-likegenes in the growth, cocoon characters and regulation of silk protein genes expression, but also provides useful information for silkworm variety breeding.

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