Occurrence and characteristics of Escherichia coli mcr-1-like in rabbits in Shandong, China

文献类型： 外文期刊

第一作者： Wang, Xinxing

作者： Wang, Xinxing;Wang, Xuepeng;Wang, Hairong;Chang, Weishan;Wang, Xinxing;Zhai, Zhenzhen;Zhao, Xiaonan;Zhang, Hongna;Jiang, Hanming

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关键词： Escherichia coli; mcr-1; plasmids; prevalence; rabbits

期刊名称：VETERINARY MEDICINE AND SCIENCE （ 影响因子：1.95； 五年影响因子：2.117 ）

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收录情况： SCI

摘要： Polymyxin is regarded as the last retort to fight against multidrug-resistant (MDR)Enterobacteriaceae. The emergency and spread of polymyxin-associated resistance genemcr-1evoked great panic of no medicine to cure the bacterial infection in society.mcr-1 is widespread in domestic and wild animals. Therefore, continuous monitoring of its prevalence and characteristics is required. In this study, we used a polymerase chain reaction (PCR)-based method to detect themcr-1 of Escherichia coliisolated from rabbits of Tai'an, China, and determined the characteristics of mcr-1-bearing plasmids. A total of 55 non-duplicatedE. coliwas recovered from the swabs of rabbit faeces. Plasmid profiling, plasmid and chromosome PCR, complete genome sequencing, a conjugation experiment, lactose fermentation experiment, multilocus sequence typing and polymyxin resistance tests were performed to determine the characteristics ofmcr-1-bearing plasmids. 14.6% (8/55) of the specimens weremcr-1 positive. Themcr-1-positiveE. coliharboured more drug-resistant genes compared with themcr-1-negative specimens, and results showed four sequence types. Overall, these findings suggested the possible threat of the transmission ofmcr-1from rabbits to humans, especially since the gene is located on transferable plasmids making horizontal transfer relatively easy. Since food-producing animals are necessary for our daily diet, worldwide cooperation is needed in fighting the spread of this drug resistance gene to avoid human infections with MDR pathogenic bacteria.

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