Genetic Diversity and Population Structure ofCannabisBased on the Genome-Wide Development of Simple Sequence Repeat Markers

文献类型： 外文期刊

第一作者： Zhang, Jiangjiang

作者： Zhang, Jiangjiang;Yan, Jiangtao;Huang, Siqi;Pan, Gen;Chang, Li;Li, Jianjun;Zhang, Chao;Tang, Huijuan;Chen, Anguo;Biswas, Ashok;Zhang, Cuiping;Zhao, Lining;Li, Defang;Huang, Siqi;Pan, Gen;Chang, Li;Li, Jianjun;Tang, Huijuan;Chen, Anguo;Zhang, Cuiping;Zhao, Lining;Li, Defang;Peng, Dingxiang

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关键词： Cannabis; genetic diversity; population structure; simple sequence repeat; cluster analysis

期刊名称：FRONTIERS IN GENETICS （ 影响因子：4.599； 五年影响因子：4.888 ）

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年卷期： 2020 年 11 卷

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收录情况： SCI

摘要： Cannabishas been used as a source of nutrition, medicine, and fiber. However, lack of genomic simple sequence repeat (SSR) markers had limited the genetic research onCannabisspecies. In the present study, 92,409 motifs were identified, and 63,707 complementary SSR primer pairs were developed. The most abundant SSR motifs had six repeat units (36.60%). The most abundant type of motif was dinucleotides (70.90%), followed by trinucleotides, tetranucleotides, and pentanucleotides. We randomly selected 80 pairs of genomic SSR markers, of which 69 (86.25%) were amplified successfully; 59 (73.75%) of these were polymorphic. Genetic diversity and population structure were estimated using the 59 (72 loci) validated polymorphic SSRs and three phenotypic markers. Three hundred ten alleles were identified, and the major allele frequency ranged from 0.26 to 0.85 (average: 0.56), Nei's genetic diversity ranged from 0.28 to 0.82 (average: 0.56), and the expected heterozygosity ranged from 0.28 to 0.81 (average: 0.56). The polymorphism information content ranged from 0.25 to 0.79 (average: 0.50), the observed number of alleles ranged from 2 to 8 (average: 4.13), and the effective number of alleles ranged from 0.28 to 0.81 (average: 0.5). TheCannabispopulation did not show mutation-drift equilibrium following analysis via the infinite allele model. A cluster analysis was performed using the unweighted pair group method using arithmetic means based on genetic distances. Population structure analysis was used to divide the germplasms into two subgroups. These results provide guidance for the molecular breeding and further investigation ofCannabis.

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