Survey of Incidence and Nested PCR Detection of Sugarcane White Leaf in Different Varieties

文献类型： 外文期刊

第一作者： Zhang, Rong-Yue

作者： Zhang, Rong-Yue;Shan, Hong-Li;Huang, Ying-Kun;Wang, Xiao-Yan;Li, Jie;Li, Wen-Feng;Cang, Xiao-Yan;Yin, Jiong;Luo, Zhi-Ming

作者机构：

关键词： phytoplasma; sugarcane white leaf; disease symptoms; incidence rate; resistant varieties

期刊名称：PLANT DISEASE （ 影响因子：4.438； 五年影响因子：4.7 ）

ISSN： 0191-2917

年卷期： 2020 年 104 卷 10 期

页码：

收录情况： SCI

摘要： Sugarcane white leaf (SCWL) is a devastating sugarcane (Saccharum officinarum) disease caused by a 16SrXI group phytoplasma, which is extremely harmful to sugarcane production. To determine the occurrence of SCWL in different varieties in 2018, we conducted a field survey and performed nested PCR detection of SCWL phytoplasma in cane-planting areas of Mangweng and Hepai in Gengma, Yunnan province, which are the areas most severely affected by SCWL in China. The results of the field survey showed that the symptomatic incidence of SCWL differed among varieties. The mean symptomatic incidence of SCWL on variety Yuetang60 was the highest (73.50%), and it was the lowest on Liucheng05-136 (13.67%). Using nested PCR, the SCWL phytoplasma was detected in symptomatic plants of all varieties more than 90% of the time; the SCWL phytoplasma was detected in 91 and 97% of symptomatic plants of Yingyu9l-59 and Liucheng05-136 varieties, respectively. The SCWL phytoplasma was detected by PCR in 82% of the asymptomatic plant samples. The results of this study showed that field survey based on white leaf symptoms did not accurately reflect the actual occurrence of the SCWL phytoplasma.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序