Transcriptome analysis reveals the roles of chlorophylla/b-binding proteins (CABs) and stay-green (SGR) in chlorophyll degradation during fruit development in kiwifruit

文献类型： 外文期刊

第一作者： Tu, Mei-yan

作者： Tu, Mei-yan;Li, Jing;Chen, Dong;Jiang, Guo-liang;Song, Hai-yan;Sun, Shu-xia;Tu, Mei-yan;Li, Jing;Chen, Dong;Jiang, Guo-liang;Song, Hai-yan;Sun, Shu-xia;Wu, Ying-ying;Yin, Xue-ren;Liu, Xiao-fen;Liu, Xiao-fen;Yin, Xue-ren;Li, Ming-zhang

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关键词： Kiwifruit; chlorophyll degradation; chlorophylla; b-binding protein; stay-green gene; transcriptome; transcription factor

期刊名称：NEW ZEALAND JOURNAL OF CROP AND HORTICULTURAL SCIENCE （ 影响因子：1.154； 五年影响因子：1.424 ）

ISSN： 0114-0671

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收录情况： SCI

摘要： Commercial cultivars of kiwifruit (Actinidiaspp.) can have green-fleshed or yellow-fleshed fruit depending on their chlorophyll degradation ability. In recent years, an increasing number of yellow-fleshed cultivars have been bred in China. However, the mechanisms underlying the flesh colouration of these new cultivars remain elusive. In this study, the pigment contents of the yellow-fleshed cultivar 'Jinshi NO.1' were determined during fruit development, and RNA sequencing was conducted to screen for differentially expressed genes and identify potential candidates for pigment metabolism. The chlorophyll content continuously decreased from 0 days after full bloom (DAFB) (T1) to 189 DAFB (T4), resulting in a more than 30-fold change, while the contents of anthocyanins and carotenoids showed sharp decreases from T1 to 44 DAFB (T2) and remained stable thereafter. Weighted gene co-expression network analysis (WGCNA) indicated that the chlorophyll contents were highly correlated with chlorophyll-degradation-related genes, especially three chlorophylla/b-binding protein (CAB) genes and one stay-green gene (AcSGR1), but no carotenoid-related genes. Furthermore, these genes were found to be highly correlated with 15 transcription factors and 11 hub genes. These results indicate the important role of chlorophyll degradation in the colouration of 'Jinshi NO.1' and provide candidates for further functional characterisation and transcriptional regulation.

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