Rv3091, An Extracellular Patatin-Like Phospholipase inMycobacterium tuberculosis, Prolongs Intracellular Survival of RecombinantMycolicibacterium smegmatisby Mediating Phagosomal Escape

文献类型： 外文期刊

第一作者： Cui, Ziyin

作者： Cui, Ziyin;Dang, Guanghui;Song, Ningning;Cui, Yingying;Li, Zhe;Zang, Xinxin;Liu, Hongxiu;Wang, Zhongxing;Liu, Siguo

作者机构：

关键词： patatin-like phospholipase; lipase; Mycobacterium tuberculosis; phagosome escape; intracellular survival

期刊名称：FRONTIERS IN MICROBIOLOGY （ 影响因子：5.64； 五年影响因子：6.32 ）

ISSN： 1664-302X

年卷期： 2020 年 11 卷

页码：

收录情况： SCI

摘要： Patatin-like phospholipases (PLPs) are important virulence factors of many pathogens. However, there are no prevailing studies regarding PLPs as a virulence factor ofMycobacterium tuberculosis(Mtb). Analysis of Rv3091, a putative protein of Mtb, shows that it belongs to the PLPs family. Here, we cloned and expressed therv3091gene inMycobacterium smegmatisand, subsequently, conducted protein purification and characterization. We show that it possesses phospholipase A(1), phospholipase A(2), and lipase activity. We confirm the putative active site residues, namely, Ser214 and Asp407, using site directed mutagenesis. The Rv3091 is an extracellular protein that alters the colony morphology ofM. smegmatis. The presence of Rv3091 enhances the intracellular survival capability ofM. smegmatisin murine peritoneal macrophages. Additionally, it promotesM. smegmatisphagosomal escape from macrophages. Moreover, Rv3091 significantly increased the survival ofM. smegmatisand aggravated lesions in C57BL/6 J murine lungsin vivo. Taken together, our results indicate that Rv3091 as an extracellular PLP that is critical to the pathogenicity of mycobacterium as it allows mycobacterium to utilize phospholipids for its growth and provides resistance to phagosome killing, resulting in its enhanced intracellular survival.

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