Comparative transcriptome analysis reveals candidate genes related to cadmium accumulation and tolerance in two almond mushroom (Agaricus brasiliensis) strains with contrasting cadmium tolerance

文献类型： 外文期刊

第一作者： Liu, Peng-Hu

作者： Liu, Peng-Hu;Chen, Li-Song;Liu, Peng-Hu;Huang, Zai-Xing;Luo, Xu-Hui;Chen, Hua;Weng, Bo-Qi;Wang, Yi-Xiang

作者机构：

期刊名称：PLOS ONE （ 影响因子：3.24； 五年影响因子：3.788 ）

ISSN： 1932-6203

年卷期： 2020 年 15 卷 9 期

页码：

收录情况： SCI

摘要： Cadmium (Cd) is a toxic metal occurring in the environment naturally. Almond mushroom (Agaricus brasiliensis) is a well-known cultivated edible and medicinal mushroom. In the past few decades, Cd accumulation inA.brasiliensishas received increasing attention. However, the molecular mechanisms of Cd-accumulation inA.brasiliensisare still unclear. In this paper, a comparative transcriptome of twoA.brasiliensisstrains with contrasting Cd accumulation and tolerance was performed to identify Cd-responsive genes possibly responsible for low Cd-accumulation and high Cd-tolerance. Using low Cd-accumulating and Cd-tolerant (J77) and high Cd-accumulating and Cd-sensitive (J1)A.brasiliensisstrains, we investigated 0, 2 and 5 mg L-1Cd-effects on mycelium growth, Cd-accumulation and transcriptome revealed by RNA-Seq. A total of 57,884 unigenes were obtained. Far less Cd-responsive genes were identified in J77 mycelia than those in J1 mycelia (e.g.,ABC transporters,ZIP Zn transporter, Glutathione S-transferase andCation efflux (CE) family). The higher Cd-accumulation in J1 mycelia might be due to Cd-induced upregulation ofZIP Zn transporter. Cd impaired cell wall, cell cycle, DNA replication and repair, thus decreasing J1 mycelium growth. Cd-stimulated production of sulfur-containing compounds, polysaccharides, organic acids, trehalose, ATP and NADPH, and sequestration of Cd might be adaptive responses of J1 mycelia to the increased Cd-accumulation. DNA replication and repair had better stability under 2 mg L-1Cd, but greater positive modifications under 5 mg L-1Cd. Better stability of DNA replication and repair, better cell wall and cell cycle stability might account for the higher Cd-tolerance of J77 mycelia. Our findings provide a comprehensive set of DEGs influenced by Cd stress; and shed light on molecular mechanism ofA.brasiliensisCd accumulation and Cd tolerance.

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