Integrated analysis of miRNA and mRNA expression profiles in testes of Duroc and Meishan boars

文献类型： 外文期刊

第一作者： Ding, Haisheng

作者： Ding, Haisheng;Liu, Min;Zhou, Changfan;You, Xiangbin;Su, Tao;Xu, Dequan;Ding, Haisheng;Liu, Min;Zhou, Changfan;You, Xiangbin;Su, Tao;Xu, Dequan;Ding, Haisheng;Yang, Youbing

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关键词： Meishan boar; Duroc boar; Testis; Sexual development; miRNA; Integrating analysis

期刊名称：BMC GENOMICS （ 影响因子：3.969； 五年影响因子：4.478 ）

ISSN： 1471-2164

年卷期： 2020 年 21 卷 1 期

页码：

收录情况： SCI

摘要： BackgroundMicroRNAs (miRNAs) are small non-coding RNAs playing vital roles in regulating posttranscriptional gene expression. Elucidating the expression regulation of miRNAs underlying pig testis development will contribute to a better understanding of boar fertility and spermatogenesis.ResultsIn this study, miRNA expression profile was investigated in testes of Duroc and Meishan boars at 20, 75, and 270days of age by high-throughput sequencing. Forty-five differentially expressed miRNAs were identified from testes of Duroc and Meishan boars before and after puberty. Integrated analysis of miRNA and mRNA profiles predicted many miRNA-mRNA pairs. Gene ontology and biological pathway analyses revealed that predicted target genes of ssc-mir-423-5p, ssc-mir-34c, ssc-mir-107, ssc-mir-196b-5p, ssc-mir-92a, ssc-mir-320, ssc-mir-10a-5p, and ssc-mir-181b were involved in sexual reproduction, male gamete generation, and spermatogenesis, and GnRH, Wnt, and MAPK signaling pathway. Four significantly differentially expressed miRNAs and their predicted target genes were validated by quantitative real-time polymerase chain reaction, and phospholipase C beta 1 (PLC beta 1) gene was verified to be a target of ssc-mir-423-5p.ConclusionsThis study provides an insight into the functional roles of miRNAs in testis development and spermatogenesis and offers useful resources for understanding differences in sexual function development caused by the change in miRNAs expression between Duroc and Meishan boars.

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