Development of an SYBR Green I-based real-time PCR assay for the rapid detection of canine kobuvirus

文献类型： 外文期刊

第一作者： Wang, Yong

作者： Wang, Yong;Cui, Yongqiu;Li, Yeqiu;Zhang, Da;Sun, Jianfei;Guo, Xu;Jiang, Shudong;Li, Yongdong;Liu, Guangqing

作者机构：

关键词： Detection; Canine kobuvirus; Real-time PCR; SYBR Green I

期刊名称：JOURNAL OF VIROLOGICAL METHODS （ 影响因子：2.014； 五年影响因子：2.001 ）

ISSN： 0166-0934

年卷期： 2020 年 285 卷

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收录情况： SCI

摘要： Canine kobuvirus (CaKoV) is a causative agent of gastroenteritis in dogs. Rapid detection of CaKoV is important for preventing and controlling this condition. In this study, an SYBR Green I-based quantitative real-time PCR assay was established for CaKoV detection. Specific primers targeting a highly conserved region of the CaKoV 3D gene were developed. After optimization, the method detected a minimum of 1 x 10(1) copies/mu L with high specificity, stability, and repeatability. Moreover, the entire process only required approximately 1.5 h for completion. Our results were supported by those obtained for clinical samples, in which our developed method was successfully applied. The newly established real-time PCR is a rapid, sensitive, specific, and repeatable method for the quantitative detection of CaKoV and can, therefore, be used in epidemiological studies.

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