Pueraria lobata Root Constituents as Xanthine Oxidase Inhibitors and Protective Agents against Oxidative Stress Induced in GES-1 Cells

文献类型： 外文期刊

第一作者： Tang, Xiaosheng

作者： Tang, Xiaosheng;Xiao, Aiping;Mei, Shiyong;Liu, Liangliang;Tang, Ping;Ren, Licheng

作者机构：

关键词： fluorescence spectra; inhibitor screening; oxidative stress; Pueraria lobata root; xanthine oxidase

期刊名称：JOURNAL OF THE BRAZILIAN CHEMICAL SOCIETY （ 影响因子：1.838； 五年影响因子：1.9 ）

ISSN： 0103-5053

年卷期： 2020 年 31 卷 10 期

页码：

收录情况： SCI

摘要： Ultrafiltration coupled with liquid chromatography-mass spectrometry (LC-MS) was established to screen xanthine oxidase (XO) inhibitors from Pueraria lobata root extract. Four compounds were screened out and identified as puerarin. daidzin, daidzein and genistein with half-maximal inhibitory concentration (IC50) values of 30.8, 5.31. 145 and 3.02 mu g mL(-1) on XO, respectively. The interactions between these compounds and XO were investigated by fluorescence spectroscopic method. The hydrogen peroxide induced oxidative stress model of human normal gastric epithelial cell lines (GES-1) was used to investigate the protections on injured cell. As a result, four XO inhibitors exhibited protective effects without cytotoxicity. With the increased concentrations of four inhibitors, cell viability was higher with decreased mortality rate, the decrease of superoxide dismutase activity, leakage of lactate dehydrogenase and increase of intracellular superoxide anion production induced by hydrogen peroxide were restrained. It showed that these four XO inhibitors could effectively enhance cell viability and protect injury of GES-1 cells from oxidative stress.

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