VdNop12, containing two tandem RNA recognition motif domains, is a crucial factor for pathogenicity and cold adaption inVerticillium dahliae

文献类型： 外文期刊

第一作者： Zhang, Jun

作者： Zhang, Jun;Cui, Weiye;Abdul Haseeb, Hafiz;Guo, Wei

作者机构：

期刊名称：ENVIRONMENTAL MICROBIOLOGY （ 影响因子：5.491； 五年影响因子：6.438 ）

ISSN： 1462-2912

年卷期：

页码：

收录情况： SCI

摘要： Previous studies have reported the ability of fungi to overwinter in soil or on crop debris under different environmental conditions, but how fungi adapt to chilling is still largely unknown. In this study, we have identified and characterized the RNA binding protein (RBP) (VdNop12) by screening anAgrobacterium tumefaciens-mediated transformation-mediated insertional mutational library ofVerticillium dahliae. We determined that this protein was essential to the pathogen for virulence on cotton plants. VdNop12 contains two tandem RNA recognition motif domains, and its orthologs are widely distributed in filamentous fungi. Mutants produced by disruption ofVdNop12showed defects in vegetative growth, conidiation and cell wall integrity. The mutant also showed an increase in sensitivity to low temperature, as compared to the wildtype and complementation strains. Yeast complementation assay showed thatVdNop12could functionally restore the growth phenotype of Delta ScNop12mutant ofSaccharomyces cerevisiaeat 15 degrees C. We demonstrated that the VdNop12 is localized in the nucleus, and its loss resulted in the downregulated expression of several genes related to cAMP-PKA and MAPK pathways inV. dahliae. Our results demonstrated a crucial role of RBPs in the regulation of morphology, cold adaption, and pathogenic development inV. dahliae.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序