Comparative transcriptome analyses provide novel insights into etiolated shoot development of walnut (Juglans regiaL.)

文献类型： 外文期刊

第一作者： Wang, Beibei

作者： Wang, Beibei;Dong, Ningguang;Chen, Yonghao;Zhang, Yunqi;Hao, Yanbin;Qi, Jianxun;Zhang, Yan;Zhang, Yan

作者机构：

关键词： Etiolation; Histology; Plant hormone; Rejuvenation; Transcriptome

期刊名称：PLANTA （ 影响因子：4.116； 五年影响因子：4.316 ）

ISSN： 0032-0935

年卷期： 2020 年 252 卷 5 期

页码：

收录情况： SCI

摘要： Main conclusion In general, genes promoting IAA, CTK GA and ethylene biosynthesis were upregulated, while genes participating in ABA, chlorophyll and starch biosynthesis pathways performed opposite tendency during etiolation. Etiolation as a method for rejuvenation plays an important role in the vegetative propagation of woody plants. However, the molecular mechanism of etiolated shoot development remains unclear. In this study, we investigated changes at different etiolation stages ofJuglans regia. The histology and transcriptome ofJ. regiawere analysed using etiolated stems, which were treated in darkness for 30, 60, 90 days. The results showed that the ratios of pith (Pi) diameter/stem diameter (D), cortex (Co) width/D, and phloem (Ph) width/Dincreased, while the ratio of xylem (Xy) width/Ddecreased after etiolation, and the difference in these ratios between etiolated stems and the control was more significant at 60 days than 90 days. Differentially expressed genes (DEGs) were significantly enriched in pathways such as plant hormone biosynthesis and signal transduction, chlorophyll biosynthesis and degradation, and starch and sucrose metabolism. The difference in the contents of indole-3-acetic acid (IAA), abscisic acid (ABA), sugar and chlorophyll between etiolated stems and the control increased with increasing treatment duration; in contrast, the concentrations of gibberellin (GA), zeatin (ZT), and starch, as well as the difference between the etiolated stems and control were lowest at 60 days among the three stages. On the whole, the positive effect of etiolation on the rejuvenation of walnut stems changed as the treatment period increased. The present investigation lays a foundation for future studies on the effect of etiolation on rejuvenation and for promoting the efficiency of vegetative propagation.

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