文献类型： 外文期刊
第一作者： Wang, Ming-Qing
作者： Wang, Ming-Qing;Zhang, Chu-Shu;Yu, Li-Na;Yang, Wei-Qiang;Jiao, Kun;Chi, Xiao-Yuan;Bi, Jie;Song, Yu;Zhang, Jian-Cheng;Sun, Jie;Zhang, Chu-Shu;Gong, Kui-Jie;Yang, Qing-Li;Yang, Gui-Wen
作者机构：
关键词： Pseudomonas laoshanensissp; nov; Bacteria; Taxonomy; Soil
期刊名称：ARCHIVES OF MICROBIOLOGY （ 影响因子：2.552； 五年影响因子：2.475 ）
ISSN： 0302-8933
年卷期：
页码：
收录情况： SCI
摘要： A novel Gram-stain-negative, aerobic strain, designated Y22(T), was isolated from peanut field soil in Laoshan Mountain in China. Cells of strain Y22(T)were rod-shaped and motile by a single flagellum. The strain was found to be oxidase- and catalase-positive. 16S rRNA gene sequence based on phylogenetic analysis indicated that strain Y22(T)belonged to the genusPseudomonas, and showed the highest 16S rRNA gene sequence similarity of 99.0% toPseudomonas pelagiaJCM 15562(T), followed byPseudomonas salinaJCM 19469(T)(98.4%),Pseudomonas sabulinigriJCM 14963(T)(97.9%),Pseudomonas bauzanensisCGMCC 1.9095(T)(97.6%) andPseudomonas litoralisKCTC23093(T)(97.5%). The phylogenetic analysis based on multilocus sequence analyses with concatenated 16S rRNA,gyrB,rpoDandrpoBgenes indicated that strain Y22(T)belonged toPseudomonas pertucinogenalineage. The average nucleotide identity scores between strain Y22(T)and closely related species were 74.6-82.8%, and the Genome-to-Genome Distance Calculator scores were 16.4-44.9%. The predominant cellular fatty acids of strain Y22(T)were C-18:1 omega 7c (29.6%), C(17:0)cyclo (17.5%) and summed feature 3 (C-16:1 omega 7c and/or C-16:1 omega 6c) (17.4%). The genomic DNA G+C content was 57.9 mol%. On the basis of phenotypic characteristics, phylogenetic analyses and in silico DNA-DNA relatedness, a novel species,Pseudomonas laoshanensissp. nov. is proposed. The type strain is Y22(T)(= JCM 32580(T) = KCTC 62385(T) = CGMCC 1.16552(T)).
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