Identification of theSUTGene Family in Pomegranate (PunicagranatumL.) and Functional Analysis ofPgL0145810.1

文献类型： 外文期刊

第一作者： Poudel, Krishna

作者： Poudel, Krishna;Luo, Xiang;Chen, Lina;Jing, Dan;Xia, Xiaocong;Tang, Liying;Li, Haoxian;Cao, Shangyin

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关键词： genetic transformation; transgene; cloning; expression analysis; cytological observation

期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：5.923； 五年影响因子：6.132 ）

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年卷期： 2020 年 21 卷 18 期

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收录情况： SCI

摘要： Sucrose, an important sugar, is transported from source to sink tissues through the phloem, and plays important role in the development of important traits in plants. However, theSUTgene family is still not well characterized in pomegranate. In this study, we first identified the pomegranate sucrose transporter (SUT)gene family from the whole genome. Then, the phylogenetic relationship ofSUTgenes, gene structure and their promoters were analyzed. Additionally, their expression patterns were detected during the development of the seed. Lastly, genetic transformation and cytological observation were used to study the function ofPgL0145810.1. A total of ten pomegranateSUTgenes were identified from the whole genome of pomegranate 'Tunisia'. The promoter region of all the pomegranateSUTgenes contained myeloblastosis (MYB) elements. Four of theSUTgenes,PgL0328370.1,PgL0099690.1,PgL0145810.1andPgL0145770.1, were differentially expressed during seed development. We further noticed thatPgL0145810.1was expressed most prominently in the stem parts in transgenic plants compared to other tissue parts (leaves, flowers and silique). The cells in the xylem vessels were small and lignin content was lower in the transgenic plants as compared to wildArabidopsisplants. In general, our result suggests that the MYB cis-elements in the promoter region might regulatePgL0145810.1expression to control the structure of xylem, thereby affecting seed hardness in pomegranate.

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