Overexpression ofCcNAC1gene promotes early flowering and enhances drought tolerance of jute (Corchorus capsularis L.)

文献类型： 外文期刊

第一作者： Zhang, Gaoyang

作者： Zhang, Gaoyang;Huang, Siqi;Zhang, Chao;Li, Defang;Zhang, Gaoyang;Wu, Yingbao;Deng, Jielou;Shan, Shilian;Qi, Jianmin

作者机构：

关键词： Jute (Corchorus capsularis L; ); NAC transcription factor; Drought stress; Yeast-2-hybrid; Molecular mechanism

期刊名称：PROTOPLASMA （ 影响因子：3.356； 五年影响因子：3.298 ）

ISSN： 0033-183X

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页码：

收录情况： SCI

摘要： Drought is the main factor that significantly affects plant growth and has devastating effects on crop production of jute. NAC (NAM, ATAF, and CUC2) transcription factors (TFs) are a large gene family in plants that have been shown to play many important roles in regulating developmental processes and abiotic stress resistance. In this study, a NAC transcription factor, CcNAC1, was cloned and characterized its function in jute. RT-qPCR analysis showed that CcNAC1 expression peaks after 8 h of drought stress. CcNAC1 overexpression and knockdown plants were created by Agrobacterium-mediated genetic transformation. PCR and southern hybridization results indicate that theCcNAC1gene was integrated into the genome of jute. Overexpression of theCcNAC1gene sped up the plant growth, promoted early flowering, and increased drought tolerance compared to the control plants. 3-Ketoacyl-CoA synthase (KCS) gene expression level increased significantly in the CcNAC1-overexpression plants and decreased in knockdown plants, which showed that CcNAC1 transcription factor regulated KCS gene expression. Yeast-2-Hybrid (Y2H) assays validated the physical interaction between CcNAC1 and KCS. The results provide relatively comprehensive information on the molecular mechanisms ofCcNAC1gene underlying the regulation of plant growth and drought stress resistance, and indicate that CcNAC1 acts as a positive regulator in drought tolerance in jute (Corchorus capsularis L.).

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