Ubiquitin-Conjugating Enzyme E2 E Inhibits the Accumulation of Rice Stripe Virus inLaodelphax striatellus(Fallen)

文献类型： 外文期刊

第一作者： Li, Yao

作者： Li, Yao;Zhou, Ze;Shen, Mi;Ge, Linquan;Liu, Fang;Li, Yao;Liu, Fang;Li, Yao

作者机构：

关键词： Laodelphax striatellus; rice stripe virus; ubiquitin-conjugating enzyme E2; viral accumulation

期刊名称：VIRUSES-BASEL （ 影响因子：5.048； 五年影响因子：5.127 ）

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年卷期： 2020 年 12 卷 9 期

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收录情况： SCI

摘要： The ubiquitin-proteasome system (UPS) is an essential protagonist in host-pathogen interactions. Among the three classes of enzymes in the UPS, ubiquitin-conjugating enzyme E2 plays a dual role in viral pathogenesis; however, the role of insect E2s in interactions with plant viruses is unclear. Twenty E2-encoding genes inLaodelphax striatellus, the small brown planthopper, were identified and classified into 17 groups by transcriptomic and phylogenetic analysis. Full-length cDNAs of fourLstrE2s(LstrE2 A/E/G2/H) were obtained by rapid-amplification of cDNA ends (RACE-PCR) analysis. Expression of the fourLstrE2sshowed tissue- and development-specific patterns. RT-qPCR analyses revealed that Rice stripe viruse (RSV) infection increased the level ofLstrE2 A/E/G2/H. Further study indicated that repression ofLstrE2 Evia RNAi caused significant increases in the expression of RSV coat protein mRNA and protein levels. These findings suggest that LstrE2 E inhibits RSV accumulation in the planthopper body. Understanding the function of LstrE2 E in RSV accumulation may ultimately result in the development of novel antiviral strategies.

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