IbERF71, withIbMYB340andIbbHLH2, coregulates anthocyanin accumulation by binding to theIbANS1promoter in purple-fleshed sweet potato (Ipomoea batatasL.)

文献类型： 外文期刊

第一作者： Ning, Zhiyuan

作者： Ning, Zhiyuan;Hu, Kangdi;Li, Lixia;Ding, Chen;Chen, Xiaoyan;Yao, Gaifang;Zhang, Hua;Zhou, Zhilin;Zhao, Donglan;Tang, Jun;Wang, Hong

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关键词： Anthocyanin biosynthesis; Purple-fleshed sweet potato (Ipomoea batatasL; ); IbERF71; IbMYB340; Regulatory complex

期刊名称：PLANT CELL REPORTS （ 影响因子：4.57； 五年影响因子：4.463 ）

ISSN： 0721-7714

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收录情况： SCI

摘要： Key message The transcription factor (TF)IbERF71forms a novel complex,IbERF71-IbMYB340-IbbHLH2, to coregulate anthocyanin biosynthesis by binding to theIbANS1promoter in purple-fleshed sweet potatoes. Purple-fleshed sweet potato (Ipomoea batatasL.) is very popular because of its abundant anthocyanins, which are natural pigments with multiple physiological functions. TFs involved in regulating anthocyanin biosynthesis have been identified in many plants. However, the molecular mechanism of anthocyanin biosynthesis in purple-fleshed sweet potatoes has rarely been examined. In this study, TFIbERF71and its partners were screened by bioinformatics and RT-qPCR analysis. The results showed that the expression levels ofIbERF71and partnersIbMYB340andIbbHLH2were higher in purple-fleshed sweet potatoes than in other colors and that the expression levels positively correlated with anthocyanin contents. Moreover, transient expression assays showed that cotransformation ofIbMYB340+IbbHLH2resulted in anthocyanin accumulation in tobacco leaves and strawberry receptacles, and additionalIbERF71significantly increased visual aspects. Furthermore, the combination of the three TFs significantly increased the expression levels ofFvANSandFvGST, which are involved in anthocyanin biosynthesis and transport of strawberry receptacles. The dual-luciferase reporter system verified that cotransformation of the three TFs enhanced the transcription activity ofIbANS1.In addition, yeast two-hybrid and firefly luciferase complementation assays revealed that IbMYB340 interacted with IbbHLH2 and IbERF71 but IbERF71 could not interact with IbbHLH2 in vitro. In summary, our findings provide novel evidence that IbERF71 and IbMYB340-IbbHLH2 form the regulatory complex IbERF71-IbMYB340-IbbHLH2 that coregulates anthocyanin accumulation by binding to theIbANS1promoter in purple-fleshed sweet potatoes. Thus, the present study provides a new regulatory network of anthocyanin biosynthesis and strong insight into the color development of purple-fleshed sweet potatoes.

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