JMJD3 acts in tandem with KLF4 to facilitate reprogramming to pluripotency

文献类型： 外文期刊

第一作者： Huang, Yinghua

作者： Huang, Yinghua;Wang, Lulu;Tang, Chuanqing;Qin, Xiaogan;Wu, Xinyu;Pan, Meifang;Tang, Yujia;Yang, Zhongzhou;Lin, Runxia;Lai, Yiwei;Xu, Xueting;Su, Jianbin;Wen, Xue;Ahmed, Tanveer;Malik, Vikas;Ward, Carl;Volpe, Giacomo;Guo, Lin;Chen, Jinlong;Huang, Xiaofen;Bao, Xichen;Zheng, Hui;Lai, Liangxue;Pan, Guangjin;Chen, Jiekai;Pei, Duanqing;Esteban, Miguel A.;Qin, Baoming;Huang, Yinghua;Wang, Lulu;Tang, Chuanqing;Qin, Xiaogan;Wu, Xinyu;Pan, Meifang;Tang, Yujia;Yang, Zhongzhou;Lin, Runxia;Lai, Yiwei;Xu, Xueting;Su, Jianbin;Ahmed, Tanveer;Malik, Vikas;Ward, Carl;Volpe, Giacomo;Guo, Lin;Chen, Jinlong;Huang, Xiaofen;Bao, Xichen;Lai, Liangxue;Pan, Guangjin;Chen, Jiekai;Pei, Duanqing;Esteban, Miguel A.;Qin, Baoming;Huang, Yinghua;Wang, Lulu;Tang, Chuanqing;Qin, Xiaogan;Wu, Xinyu;Pan, Meifang;Tang, Yujia;Lin, Runxia;Xu, Xueting;Su, Jianbin;Ahmed, Tanveer;Qin, Baoming;Zhang, Hui;Li, Yingying;Bao, Xichen;Zheng, Hui;Lai, Liangxue;Pei, Duanqing;Qin, Baoming;Wu, Xinyu;Pan, Meifang;Lin, Runxia;Babarinde, Isaac A.;Sun, Li;Hutchins, Andrew P.;Ji, Guanyu;Gao, Fei;Lai, Yiwei;Malik, Vikas;Ward, Carl;Volpe, Giacomo;Esteban, Miguel A.;Xu, Xueting;Wen, Xue;Hu, Jifan;Satoh, Takashi;Akira, Shizuo;Bao, Xichen;Zheng, Hui;Lai, Liangxue;Pan, Guangjin;Chen, Jiekai;Esteban, Miguel A.;Qin, Baoming;Bao, Xichen;Zheng, Hui;Lai, Liangxue;Pan, Guangjin;Chen, Jiekai;Esteban, Miguel A.;Qin, Baoming;Gao, Fei;Liu, Baohua;Jauch, Ralf;Testa, Giuseppe;Esteban, Miguel A.

作者机构：

期刊名称：NATURE COMMUNICATIONS （ 影响因子：14.919； 五年影响因子：15.805 ）

ISSN： 2041-1723

年卷期： 2020 年 11 卷 1 期

页码：

收录情况： SCI

摘要： The interplay between the Yamanaka factors (OCT4, SOX2, KLF4 and c-MYC) and transcriptional/epigenetic co-regulators in somatic cell reprogramming is incompletely understood. Here, we demonstrate that the histone H3 lysine 27 trimethylation (H3K27me3) demethylase JMJD3 plays conflicting roles in mouse reprogramming. On one side, JMJD3 induces the pro-senescence factor Ink4a and degrades the pluripotency regulator PHF20 in a reprogramming factor-independent manner. On the other side, JMJD3 is specifically recruited by KLF4 to reduce H3K27me3 at both enhancers and promoters of epithelial and pluripotency genes. JMJD3 also promotes enhancer-promoter looping through the cohesin loading factor NIPBL and ultimately transcriptional elongation. This competition of forces can be shifted towards improved reprogramming by using early passage fibroblasts or boosting JMJD3's catalytic activity with vitamin C. Our work, thus, establishes a multifaceted role for JMJD3, placing it as a key partner of KLF4 and a scaffold that assists chromatin interactions and activates gene transcription. Previous work suggested that histone demethylase JMJD3 is detrimental to somatic cell reprogramming. Here, the authors show that while JMJD3 has a context-independent detrimental effect on early stages of reprogramming, during late stages it activates epithelial and pluripotency genes together with Klf4.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序