Profiling of rice Cd-tolerant genes through yeast-based cDNA library survival screening

文献类型： 外文期刊

第一作者： Wang, Baoxiang

作者： Wang, Baoxiang;Zhang, Manman;Zhang, Jie;Chen, Xi;Jiang, Mingyi;Tan, Mingpu;Huang, Liping

作者机构：

关键词： Cd tolerance; BAX inhibitor; Rapid ALkalinization factors; NAC transcription Factor; HMAD isoprenylated Protein; Rice

期刊名称：PLANT PHYSIOLOGY AND BIOCHEMISTRY （ 影响因子：4.27； 五年影响因子：4.816 ）

ISSN： 0981-9428

年卷期： 2020 年 155 卷

页码：

收录情况： SCI

摘要： The bioaccumulation of cadmium (Cd) in crop and the subsequent food chain has aroused extensive concerns. However, the underlying molecular mechanisms of plant Cd tolerance remain to be clarified from the viewpoint of novel candidate genes. Here we described a highly efficient approach for preliminary identifying rice Cd-tolerant genes through the yeast-based cDNA library survival screening combined with high-throughput sequencing strategy. About 690 gene isoforms were identified as being Cd-tolerant candidates using this shotgun approach. Among the Cd-tolerant genes identified, several categories of genes such as BAX inhibitor (BI), NAC transcription factors and Rapid ALkalinization Factors (RALFs) were of particular interest, and their function of Cd tolerance was further validated via heterologous expression, which suggested that SNACI, RALF12, OsBI-1 can confer Cd tolerance in yeast and tobacco leaves. Regarding the genes involved in ion transport, the validated Cd-tolerant heavy metal-associated domain (HMAD) isoprenylated protein HIPP42 was particularly noteworthy. Further elucidation of these genes associated with Cd tolerance in rice will benefit agricultural activities.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序