Effects of changing temperature on the physiological and biochemical properties of Harmonia axyridis larvae

文献类型： 外文期刊

第一作者： Wang, Shasha

作者： Wang, Shasha;Li, Yan;Pan, Biying;Wang, Shigui;Tang, Bin;Chen, Xu;Wang, Su;Dai, Huijie

作者机构：

关键词： Coccinellidae; trehalose metabolism; glycerol; RT-qPCR

期刊名称：ENTOMOLOGIA GENERALIS （ 影响因子：5.625； 五年影响因子：4.207 ）

ISSN： 0171-8177

年卷期： 2020 年 40 卷 3 期

页码：

收录情况： SCI

摘要： Harmonia axyridis (Pallas) is an important natural enemy insect. Using fourth-instar larvae, we investigated whether changing temperature (5, 15 and 25 degrees C) can enhance the cold tolerance of H. axyridis and determined optimal storage temperature conditions. Larvae were exposed to five altering temperature regimes:15/5 (15 degrees C/12 h:5 degrees C/24 h), 5/15/5 (5 degrees C/12h:15 degrees C/12h:5 degrees C/24h),15/25/5(15 degrees C/12h:25 degrees C/12h:5 degrees C/24h),15/5/25/5(15 degrees C/12h:5 degrees C/12h:25 degrees C/12h:5 degrees C/24h), and 5/25/15/5 (5 degrees C/12 h:25 degrees C/12 h:15 degrees C/12 h:5 degrees C/24 h). Compared with the larvae of control treatment (5 degrees C/24 h), the larvae had lower supercooling points (SCPs) in the 15/25/5 and 5/25/15/5 groups, higher glycerol content in all treated groups (except for group 5/15/5), lower fat content in the 15/5/25/5 group, lower water content in the 15/25/5 and 15/5/25/5 groups, higher trehalose content in the 15/5, 15/25/5, and 15/5/25/5 groups, higher glucose content in the 15/25/5 group, and higher glycogen content in the 15/25/5, 15/5/25/5, and 5/25/15/5 groups. Compared with the control larvae, TRE1 activity of all treated larvae was significantly enhanced except for those in the 5/15/5 group. Also, the expression levels of three soluble trehalase genes (TRE1-1, TRE1-2 and TRE1-3) and one membrane-bound trehalase gene (TRE2) increased after the changing temperature treatment, whereas TRE1-4, TRE1-5, and one membrane-bound like trehalase gene (TRE2-like) mRNA expression levels were very low. These results suggest that fourth-instar H. axyridis larvae exposed to a succession of different temperatures, including low temperatures, survived by reducing the SCP, accumulating carbohydrates and glycerol, and consuming fat. The temperature combination 15/25/5 degrees C provided optimal storage conditions. These findings provide valuable insights for further elucidation of the cold resistance mechanism of ladybeetles and for obtaining an extended shelf life under low-temperature storage.

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