Gracilibacillus oryzae sp. nov., isolated from rice seeds

文献类型： 外文期刊

第一作者： He, Shan-Wen

作者： He, Shan-Wen;Wang, Xing;Guo, He-Bao;Ma, Li-An;He, Shan-Wen;Thin, Kyu Kyu;Gao, Ju-Sheng;Zhang, Xiao-Xia;Han, Ji-Gang;Gao, Ju-Sheng

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期刊名称：INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY （ 影响因子：2.747； 五年影响因子：2.889 ）

ISSN： 1466-5026

年卷期： 2020 年 70 卷 10 期

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收录情况： SCI

摘要： A Gram-stain-positive, facultatively anaerobic, endospore-forming bacterium, designated strain TD8(T), was isolated from surface-sterilized rice seeds (Oryza sativa L.). Phylogenetic analysis of the 16S rRNA gene indicated that strain TD8(T) should be placed within the genus Gracilibacillus (95.2-99.0% sequence similarity); it exhibited highest similarities to Gracilibacillus ureilyticus CGMCC 1.7727(T) (99.0 %), 'Gracilibacillus xinjiangensis' CGMCC 1.12449(T) (98.9%) and Gracilibacillus dipsosauri CGMCC 1.3642(T) (97.5 %). Chemotaxonomic analysis showed that menaquinone-7 (MK-7) was the major isoprenoid quinone. Diphosphatidylglycerol, phosphatidylglycerol and one unidentified phospholipid were the major cellular polar lipids, and the major fatty acids were anteiso-C-15:0, anteiso-C-17:0, iso-C-15:0, C-16:0 and iso-C-16:0, which supported the allocation of the strain to the genus Gracilibacillus. The digital DNA-DNA hybridization value between strain TD8(T) and Gracilibacillus ureilyticus CGMCC 1.7727(T) was lower than 70 % (22.60 %), and the average nucleotide identity score was 79.54 +/- 5.09%, suggesting that strain TD8(T) represented a novel species in the genus Gracilibacillus. The genomic DNA G+C content was 37.5 %. Based on physiological and biochemical characteristics and genotypic data, strain TD8(T) represents a novel species of the genus Gracilibacillus, for which the name Gracilibacillus oryzae sp. nov. is proposed. The type strain is TD8(T) (=ACCC 61556(T)=CICC 24889(T)=JCM 33537(T)).

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