Analyzing Differentially Expressed Genes and Pathways Associated with Pistil Abortion in Japanese Apricot via RNA-Seq

文献类型： 外文期刊

第一作者： Shi, Ting

作者： Shi, Ting;Iqbal, Shahid;Bai, Yang;Pan, Zhenpeng;Ni, Xiaopeng;Hayat, Faisal;Gao, Zhihong;Iqbal, Shahid;Ayaz, Aliya;Saqib Bilal, Muhammad;Khuram Razzaq, Muhammad

作者机构：

关键词： pistil abortion; Japanese apricot; RNA-Seq; hormone signaling; metabolic pathways

期刊名称：GENES （ 影响因子：4.096； 五年影响因子：4.339 ）

ISSN：

年卷期： 2020 年 11 卷 9 期

页码：

收录情况： SCI

摘要： Reproduction is a critical stage in the flower development process, and its failure causes serious problems affecting fruit quality and yield. Pistil abortion is one of the main factors in unsuccessful reproduction and occurs in many fruit plants. In Japanese apricot, the problem of pistil abortion is very common and affects fruit quality and plant yield; however, its molecular mechanism is not clearly understood. Therefore, in the current study, we used RNA-Seq to identify the differentially expressed genes (DEGs) and pathways actively involved in pistil abortion. A total of 3882 differentially expressed genes were found after cutoff and pairwise comparison analysis. According to KEGG pathway analysis, plant hormone signaling transduction and metabolic pathways were found most significantly enriched in this study. A total of 60 transcription factor families such as MADS-box, NAC and TCP showed their role in this process. RT-qPCR assays confirmed that the expression levels were consistent with RNA-Seq results. This study provides an alternative to be considered for further studies and understanding of pistil abortion processes in Japanese apricot, and it provides a reference related to this issue for other deciduous fruit crops.

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