Development of a real-time recombinase polymerase amplification assay for rapid detection of Aeromonas hydrophila

文献类型： 外文期刊

第一作者： Qu, Yang

作者： Qu, Yang;Wang, Qing;Li, Yingying;Wang, Yingying;Yin, Jiyuan;Ren, Yan;Liu, Xiaofang;Wang, Yahui;Zeng, Weiwei;Qu, Yang;Liu, Xiaofang;Liu, Chun;Zeng, Weiwei

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关键词： Aeromonas hydrophila; detection; haemolysin gene; real‐ time recombinase polymerase amplification

期刊名称：JOURNAL OF FISH DISEASES （ 影响因子：2.767； 五年影响因子：2.689 ）

ISSN： 0140-7775

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收录情况： SCI

摘要： Aeromonas hydrophila is ubiquitous in the aquaculture industry and a constant cause of severe disease and economic losses. The early diagnosis of these infections is crucial for disease surveillance and prevention. We developed a real-time recombinase polymerase amplification (real-time RPA) assay for detection of A. hydrophila using the haemolysin gene. The assay was performed at 37 degrees C for 20 min and was highly specific with no cross-reaction with other fish pathogens or with other Aeromonas species. The assay detection limit was 10(2) copies of the Aeromonas hydrophila per reaction. Compared with traditional culture-based method or real-time PCR, the diagnostic sensitivity and specificity of the real-time RPA were 73.7 and 100%, as well as 64.7 and 93%. Our newly developed real-time RPA was specific and sensitive and can be used in large-scale and point-of-care field investigations of A. hydrophila infections to enable earlier diagnoses.

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