Analysis of the Effect of Different Reducing Sugars on Ara h2 Glycation Based on Spectral Technology

文献类型： 外文期刊

第一作者： Yang, Ping

作者： Yang, Ping;Wang, Hui;Li, Xue;Liu, Guang-xian

作者机构：

关键词： Glycation; Ara h2; Spectroscopic techniques; Reducing sugar

期刊名称：SPECTROSCOPY AND SPECTRAL ANALYSIS （ 影响因子：0.7； 五年影响因子：0.6 ）

ISSN： 1000-0593

年卷期： 2023 年 43 卷 4 期

页码：

收录情况： SCI

摘要： Glycation reaction can induce the structural change of protein in food stuff; Ara h2 is one of the main proteins in peanuts, and it can be used as a model protein to study the structural change of the glycation products of peanut protein. However, the effects of different reducing sugars on Ara h2 glycation have not been reported. Therefore, this article took Ara h2 as the research object to study the changes in the molecular weight, the secondary and tertiary structure and the functional groups of Ara h2 before and after glycation by SDS-PAGE, endogenous fluorescence, synchronous fluorescence, ultraviolet spectrum, circular dichroism, Fourier transform infrared spectroscopy and other spectroscopic techniques. The effects of six reducing sugars (ribose, xylose, galactose, glucose, fructose and lactose) on Ara h2 were analyzed to clarify the structural change of different Ara h2 glycation products. The results of SDS-PAGE showed that these electrophoretic bands of Ara h2 modified by xylose and ribose moved up significantly, and their glycation degree was the largest, compared with other reducing sugars. Ultraviolet spectrum analysis showed that glycation reaction would change the absorption peak intensity of Ara h2, and modification with pentoses had the strongest absorption intensity (absorption peak intensity of xylose was the largest). The results of endogenous fluorescence, synchronous fluorescence and three-dimensional spectral scanning showed that glycation reduced the fluorescence intensity of Ara h2 and pentose modified Ara h2 had the lowest fluorescence intensity. It might be due to the structural unfolding of Ara h2 caused by glycation, which exposes aromatic amino acids to the water environment and leads to fluorescence quenching. Circular dichroism analysis showed that the content of a-helix increased after Ara h2 was modified by different reducing sugars, among which modified by xylose showed the highest helix content (15. 60 o). Fourier transform infrared spectroscopy showed that the absorption peaks of Ara h2 (modified by xylose and ribose) shifted from 3 327. 41 to 3 318. 43 and 3 321. 09 cm(-1), respectively; At 1 700 similar to 1 600 cm(-1), the absorption peak intensity of Ara h2 modified by xylose and ribose was slightly higher than that modified by other reducing sugars. Therefore, different reducing sugars have different effects on the structure of Ara h2 glycation products; The shorter carbon chain and the less steric hindrance of reducing sugars led to a higher glycation degree and a greater impact on the structure of Ara h2.

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