The Peripherin Gene Regulates the Migration of Bone Marrow Mesenchymal Stem Cells in Wuzhishan Mini Pigs

文献类型： 外文期刊

第一作者： Feng, Zheng

作者： Feng, Zheng;Li, Wentong;Yu, Hui;Li, Hua;Li, Kui;Li, Wentong;Xia, Ying;Li, Kui;Mu, Yulian

作者机构：

期刊名称：STEM CELLS INTERNATIONAL （ 影响因子：5.443； 五年影响因子：5.305 ）

ISSN： 1687-966X

年卷期： 2020 年 2020 卷

页码：

收录情况： SCI

摘要： Increasing the migratory capacity of the implanted mesenchymal stem cells (MSCs) is a major challenge in developing successful cell transplantation therapies. Nevertheless, the regulatory factors involved in the migration of BMMSCs remain largely unknown. In this study, we studied the role of the peripherin (PRPH) gene in regulating the ability of Wuzhishan mini pig (WZSP) BMMSCs to migrate in vitro. Four different shRNA vectors directed against PRPH were designed and transfected into BMMSCs. The vector with the best interference effect was chosen to be used in the following experiments. The expression level of PRPH in BMMSCs was determined by quantitative real-time PCR and western blot analysis. The migration capacity of the BMMSCs was estimated using a scratch assay, a transwell in vitro migration model assay, and filamentous actin staining. The results showed that shRNA-mediated knockdown of the expression of the PRPH gene in BMMSCs reduced the ability of these cells to migrate. Overall, these results illustrate that the PRPH gene regulates the migration of BMMSCs in the WZSP.

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