文献类型： 外文期刊
第一作者： Yin, L.
作者： Yin, L.;Chen, M. X.;Zeng, T. H.;Huang, R. Q.;Yin, L.;Huang, R. Q.;Liu, X. M.;Zhu, F.
作者机构：
关键词： Bacillus coagulans; high yield; optimization; rice straw hydrolysate; spore
期刊名称：LETTERS IN APPLIED MICROBIOLOGY （ 影响因子：2.858； 五年影响因子：2.776 ）
ISSN： 0266-8254
年卷期：
页码：
收录情况： SCI
摘要： Spore-forming Bacillus sp. has been extensively studied for their probiotic properties. In this study, an acid-treated rice straw hydrolysate was used as carbon source to produce the spores of Bacillus coagulans. The results showed that this hydrolysate significantly improved the spore yield compared with other carbon sources such as glucose. Three significant medium components including rice straw hydrolysate, MnSO4 and yeast extract were screened by Plackett-Burman design. These significant variables were further optimized by response surface methodology (RSM). The optimal values of the medium components were rice straw hydolysate of 27% (v/v), MnSO4 of 0 center dot 78 g l(-1) and yeast extract of 1 center dot 2 g l(-1). The optimized medium and RSM model for spore production were validated in a 5 l bioreactor. Overall, this sporulation medium containing acid-treated rice straw hydrolysate has a potential to be used in the production of B. coagulans spores.
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