Molecular cloning and functional characterization of a thioredoxin peroxidase gene in Echinococcus multilocularis

文献类型： 外文期刊

第一作者： Guo, Xiaola

作者： Guo, Xiaola;Zhang, Junmei;Li, Rui;Li, Hong;He, Xuedong;Wang, Shuai;Zheng, Yadong;Guo, Xiaola;Wang, Zhengrong;Harandi, Majid Fasihi;Hu, Junjie;Zheng, Yadong

作者机构：

关键词： Echinococcus multilocularis; Thioredoxin peroxidase; Peroxidase activity; Oxidative stress; M2 macrophage

期刊名称：MOLECULAR AND BIOCHEMICAL PARASITOLOGY （ 影响因子：1.759； 五年影响因子：2.135 ）

ISSN： 0166-6851

年卷期： 2021 年 245 卷

页码：

收录情况： SCI

摘要： Thioredoxin peroxidase (TPx) plays an important role in protecting parasites against oxidative damage. However, studies on the role of TPxs in Echinococcus multilocularis are limited. In this study, one tpx gene of E. multilocularis, named as emtpx-1, was identified. EmTPx-1 shares two positionally conserved cysteine residues (Cys48 and Cys169) with orthologs from other platyhelminths. EmTPx-1 is highly expressed in the germinal layer and present in exosome-like vesicles secreted by E. multilocularis metacestodes. EmTPx-1 displays peroxidase activity, which removes hydrogen peroxide in the presence of dithiothreitol. Furthermore, EmTPx-1 could protect DNA from oxidative damages, and EmTPx-1-expressing E. coli cells had an enhanced resistance to oxidative stress. In addition, EmTPx-1 enhanced the expression of arg1, ym1, and il-10, but suppressed inos, tnf-alpha, and il-1 beta expression in LPS-stimulated macrophages. Our data suggest a critical role for EmTPx-1 in oxidative stresses and M2 macrophage polarization.

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