BSA-seq and quantitative trait locus mapping reveals a major effective QTL for carpel number in watermelon (Citrullus lanatus)

文献类型： 外文期刊

第一作者： Qiu, Boyan

作者： Qiu, Boyan;Zhang, Taifeng;Zhu, Zicheng;Luan, Feishi;Gao, Peng;Liu, Shi;Qiu, Boyan;Zhang, Taifeng;Zhang, Shuqing;Qu, Qianrui;Zhu, Zicheng;Luan, Feishi;Gao, Peng;Liu, Shi;Qiu, Boyan;Liu, Shusen;Song, Zhengfeng;Xia, Lianqin;Yang, Zhongzhou;Zhang, Qian

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关键词： BSA-seq; CAPS; carpel number; mapping; watermelon

期刊名称：PLANT BREEDING （ 影响因子：2.536； 五年影响因子：2.306 ）

ISSN： 0179-9541

年卷期： 2022 年 141 卷 3 期

页码：

收录情况： SCI

摘要： The carpel number (CN) is related to fruit shape variation in watermelon and represents one of the most important agronomic traits. In this study, ZXG01553 (5-CN type) and W1-17 (3-CN type) lines were used as parental material to construct F-1, F-2 and backcross populations for genetic inheritance analysis and QTL (quantitative trait locus) mapping. The F-1 generation displayed the 3-CN type and the frequency distribution of F-2 individuals showed partial separation suggesting that the 3-CN was dominant to 5-CN trait and controlled by a major gene. Bulked-segregant analysis (BSA-seq) identified a chromosome segment related to the CN trait spanning 2.44 Mb on chromosome 7. Genetic linkage analysis using QTL mapping software narrowed this region to 244 Kb, and a major effective QTL was detected between markers Chr07-30681552 and Chr07-30923898. Twenty-four genes were identified in the QTL region, in which Cla97C07G143260 annotated as Receptor-like protein kinase 2 was the most likely candidate gene for the CN trait in watermelon. Our research provides new insights into the forward genetics of CN variation in cucurbit crops.

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