Synthesis and evaluation of novel tetrahydroisoquinoline-benzo[h] chromen-4-one conjugates as dual ABCB1/CYP1B1 inhibitors for overcoming MDR in cancer

文献类型： 外文期刊

第一作者： Dong, Jinyun

作者： Dong, Jinyun;Li, YuLong;Jin, Zhiyuan;Wu, Zumei;Cai, Maohua;Pan, Guangzhao;Qin, Jiang-Jiang;Ye, Wenchong;Zhou, Wen;Li, Zheshen;Chen, Zhe-Sheng;Tian, Sichao

作者机构：

关键词： Cancer; Inhibitor; ABCB1; MDR

期刊名称：BIOORGANIC & MEDICINAL CHEMISTRY （ 影响因子：3.0； 五年影响因子：3.2 ）

ISSN： 0968-0896

年卷期： 2024 年 114 卷

页码：

收录情况： SCI

摘要： The emergence of multidrug resistance (MDR) in malignant tumors is one of the major threats encountered currently by many chemotherapeutic agents. Among the various mechanisms involved in drug resistance, Pglycoprotein (P-gp, ABCB1), a member of the ABC transporter family that significantly increases the efflux of various anticancer drugs from tumor cells, and the metabolic enzyme CYP1B1 are widely considered to be two critical targets for overcoming MDR. Unfortunately, no MDR modulator has been approved by the FDA to date. In this study, based on pharmacophore hybridization, bioisosteric and fragment-growing strategies, we designed and synthesized 11 novel tetrahydroisoquinoline-benzo[h]chromen-4-one h ]chromen-4-one conjugates as dual ABCB1/CYP1B1 inhibitors. Among them, the preferred compound A10 exhibited the best MDR reversal activity (IC50 50 = 0.25 mu M, RF = 44.4) in SW620/AD300 cells, being comparable to one of the most potent third-generation P-gp inhibitors WK-X-34. In parallel, this dual ABCB1/CYP1B1 inhibitory effect drives compound A10 exhibiting prominent drug resistance reversal activity to doxorubicin (IC50 50 = 4.7 mu M, RF = 13.7) in ABCB1/CYP1B1-overexpressing DOX-SW620/AD300-1B1 resistant cells, which is more potent than that of the CYP1B1 inhibitor ANF. Furthermore, although compound A2 possessed moderate ABCB1/CYP1B1 inhibitory activity, it showed considerable antiproliferative activity towards drug-resistant SW620/AD300 and MKN45-DDP-R cells, which may be partly related to the increase of PUMA expression to promote the apoptosis of the drug-resistant MKN45-DDP-R cells as confirmed by proteomics and western blot assay. These results indicated that the tetrahydroisoquinoline-benzo [h]chromen-4-one h ]chromen-4-one conjugates may provide a fundamental scaffold reference for further discovery of MDR reversal agents.

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