Effect of Different Extraction Methods on Physicochemical Characteristics and Antioxidant Activity of C-Phycocyanin from Dry Biomass of Arthrospira platensis
文献类型: 外文期刊
第一作者: Chen, Qian
作者: Chen, Qian;Xiong, Hua;Zhao, Qiang;Li, Shuhui
作者机构:
关键词: C-phycocyanin; high-pressure cell disruption; antioxidant activity; extraction method; Arthrospira platensis
期刊名称:FOODS ( 影响因子:5.561; 五年影响因子:5.94 )
ISSN:
年卷期: 2022 年 11 卷 9 期
页码:
收录情况: SCI
摘要: The effect of four different extraction methods on physicochemical characteristics and functionalities of chloro-phycocyanin (CP) was investigated. Swelling (S-CP), freezing and thawing (4FT-CP), ultrasonication with freezing and thawing (4FT+U-CP), and the high-pressure cell disruption (HPCD-CP) process affected CP differently, thus resulting in different levels of solubility, DPPH scavenging activity, ABTS scavenging activity, and reducing power. Among the four CPs, HPCD-CP had the highest CP content (15.3%), purity (1.66 +/- 0.16), and Delta E value but the lowest Delta b value. The zeta potential of HPCD-CP (-38.8 mV) was the highest, but the average particle size of 4FT+U-CP (719.1 nm) was the highest. UV-Vis absorption spectra and fluorescence spectra illustrated that high-pressure cell disruption-assisted extraction had more profound impacts on the microenvironment of tetrapyrrole chromophores, the environment of aromatic amino acids, and the phycocyanobilin of CP. Furthermore, HPCD-CP and 4FT-CP showed higher solubility and antioxidant activities than S-CP, especially 4FT+U-CP. The results obtained in this study demonstrate that HPCD technology could obtain a food-grade C-phycocyanin product with higher CP concentration, purity, solubility, and antioxidant activity.
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