Glutaredoxin 2 in the mud crab Scylla paramamosain: Identification and functional characterization under hypoxia and pathogen challenge

文献类型： 外文期刊

第一作者： Jie, Yu-Kun

作者： Jie, Yu-Kun;Ma, Hong-Ling;Jiang, Jian-Jun;Cheng, Chang-Hong;Deng, Yi-Qin;Liu, Guang-Xin;Fan, Si-Gang;Guo, Zhi-Xun;Jie, Yu-Kun

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关键词： Glutaredoxin 2; Scylla paramamosain; Pathogen challenge; Hypoxia; Reactive oxygen species

期刊名称：DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY （ 影响因子：2.9； 五年影响因子：3.1 ）

ISSN： 0145-305X

年卷期： 2023 年 143 卷

页码：

收录情况： SCI

摘要： Glutaredoxin (Grx) is a glutathione-dependent oxidoreductase that plays a key role in antioxidant defense. In this study, a novel Grx2 gene (SpGrx2) was identified from the mud crab Scylla paramamosain, which consists of a 196 bp 5 & PRIME; untranslated region, a 357 bp open reading frame, and a 964 bp 3 & PRIME; untranslated region. The putative SpGrx2 protein has a typical single Grx domain with the active center sequence C-P-Y-C. The expression analysis revealed that the SpGrx2 mRNA was most abundant in the gill, followed by the stomach and hemocytes. Both mud crab dicistrovirus-1 and Vibrio parahaemolyticus infection as well as hypoxia could differentially induce the expression of SpGrx2. Furthermore, silencing SpGrx2 in vivo affected the expression of a series of antioxidant -related genes after hypoxia treatment. Additionally, SpGrx2 overexpression significantly increased the total antioxidant capacity of Drosophila Schneider 2 cells after hypoxia, resulting in a reduction of reactive oxygen species and malondialdehyde content. The subcellular localization results indicated that SpGrx2 was localized in both the cytoplasm and the nucleus of Drosophila Schneider 2 cells. These results indicate that SpGrx2 plays a crucial role as an antioxidant enzyme in the defense system of mud crabs against hypoxia and pathogen challenge.

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