An Oryza-specific hydroxycinnamoyl tyramine gene cluster contributes to enhanced disease resistance

文献类型： 外文期刊

第一作者： Shen, Shuangqian

作者： Shen, Shuangqian;Peng, Meng;Wang, Zixuan;Jing, Xinyu;Zhang, Meng;Yang, Chenkun;Li, Yufei;Yuan, Meng;Shen, Shuangqian;Peng, Meng;Wang, Zixuan;Jing, Xinyu;Zhang, Meng;Yang, Chenkun;Li, Yufei;Yuan, Meng;Zhou, Shen;Guo, Hao;Lei, Long;Shi, Yuheng;Sun, Yangyang;Liu, Xianqing;Xu, Congping;Luo, Jie;Fang, Hong;Ning, Yuese;Wang, Guo-Liang;Peng, Meng;Fernie, Alisdair R.;Fernie, Alisdair R.;Tohge, Takayuki

作者机构：

关键词： Rice; Gene cluster; Hydroxycinnamic acid amide; Phytoalexin; Xanthomonas oryzae pv. oryzae; Magnaporthe oryzae; Transposon element

期刊名称：SCIENCE BULLETIN （ 影响因子：20.577； 五年影响因子：12.624 ）

ISSN： 2095-9273

年卷期： 2021 年 66 卷 23 期

页码：

收录情况： SCI

摘要： Genomic clustering of non-homologous genes for the biosynthesis of plant defensive compounds is an emerging theme, but insights into their formation and physiological function remain limited. Here we report the identification of a newly discovered hydroxycinnamoyl tyramine (HT) gene cluster in rice. This cluster contains a pyridoxamine 5'-phosphate oxidase (OsPDX3) producing the cofactor pyridoxal 5'-phosphate (PLP), a PLP-dependent tyrosine decarboxylase (OsTyDC1), and two duplicated hydroxycinnamoyl transferases (OsTHT1 and OsTHT2). These members were combined to represent an enzymological innovation gene cluster. Natural variation analysis showed that the abundance of the toxic tyramine intermediate of the gene cluster among different rice accessions is mainly determined by the coordinated transcription of OsTyDC1 and OsTHT1. Further pathogen incubation assays demonstrated that the end products of the HT gene cluster displayed enhanced resistance to the bacterial pathogen Xanthomonas oryzae pv. Oryzae (Xoo) and fungal pathogen Magnaporthe oryzae (M. oryzae), and the enhanced resistance is associated with the boost of phytoalexins and the activation of defense response. The unique presence of the HT gene cluster in Oryza AA genome, together with the enrichment of transposon elements within this gene cluster region, provides an evolutionary background to accelerate cluster member combinations. Our study not only discovered a gene cluster involved in the phenylpropanoid metabolism but also addressed the key aspects of gene cluster formation. In addition, our results provide a new metabolic pool for plant defense against pathogens. (C) 2021 Science China Press. Published by Elsevier B.V. and Science China Press. All rights reserved.

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